SNPs analysis in the CDKN2A CDKN2B genes in human assayed by DNA sequencing and ARMS™ testing

الملخص EN

This research facilitates the detection process of single-nucleotide polymorphism (SNP) by DNA sequencing and Amplification Refractory Mutation System™ (ARMS™).

Alterations of four SNPs have been investigated at the specific location in CDKN2A and CDKN2B genes.

In fact, these are tumor suppressor genes.

The DNA from the cultured cell were extracted and then by using specific Oligonucleotide for SNPs, the CDKN2A and CDKN2B genes were amplified by PCR later separated by gel electrophoresis.

Finally, the ARMS test and sequencing test were applied for all samples to find out allele frequency and genotype frequency in this population.

The result presented that the efficient selection of oligonucleotides able to discriminate a single nucleotide mismatch also shows that different people may have different SNP on the same region in the chromosome.

These data will facilitate the design of custom made primer viable to gene expression profiling and sequencing analyses even it will be very interesting to compare the Kurdish People genomes to find the correlation between the SNP with several genetic backgrounds diseases.

Both results ARMS and sequencing test have compared together.

It was illustrated that there are different in genotype results by ARMS test and sequencing test for the same sample.

After the sequencing stage, it was concluded that ARMs test can be used for sequencing the genomes of a large number of people, additionally it is very sensitive, inexpensive and rapid to find out different SNPs of each individual and generate a single map of the human genome containing all possible SNPs (SNP maps).

Depending on this map we can group the people based on the SNP profile to determine if SNP associated with a disease, also explain why some people respond to treatment and not others?