Effects of folic acid on the antiproliferative efficiency of doxorubicin, camptothecin and methyl methanesulfonate in MCF-7 cells by mRNA endpoints
المؤلفون المشاركون
Xavier, Muriel Almeida
Oliveira, Marcelo Tempesta de
Baranoski, Adrivanio
Mantovani, Mario Sergio
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 25، العدد 8 (31 ديسمبر/كانون الأول 2018)، ص ص. 1568-1576، 9ص.
الناشر
تاريخ النشر
2018-12-31
دولة النشر
السعودية
عدد الصفحات
9
التخصصات الرئيسية
الموضوعات
الملخص EN
There is a lack of consensus on whether the role of folate in cancer cells is protective or harmful.
The use of folates in combination with cancer-targeting therapeutic regimens requires detailed information to ensure their safe and proper use.
Therefore, we evaluated the effects of folic acid (FA) in combination with the chemotherapeutic compounds doxorubicin (DXR), camptothecin (CPT) and methyl methanesulfonate (MMS) on the growth of MCF-7 cells.
The data generated from the RTCA assays demonstrated that FA did not affect proliferation in MCF-7 cells treated with DXR and CPT; however, FA reduced the efficacy of MMS treatment.
RTCA data also confirmed that DXR and CPT exert their cytotoxic effects in a time-dependent manner and that CPT induced a significantly greater decrease in MCF-7 cell proliferation compared with DXR.
The MTT assay failed to detect a reduction in cell proliferation in cells treated with MMS.
We quantified the mRNA expression levels of genes associated with cellular stress response, cell cycle and apoptosis pathways using RT-qPCR.
The addition of FA to DXR or CPT promoted a similar shift in the gene expression profile of MCF-7 cells compared with cells treated with DXR or CPT without FA; however, this shift did not alter the bioactivity of these drugs.
Rather, it indicated that these drugs promoted cell death by alternative mechanisms.
In contrast, the addition of FA to MMS reduced the efficacy of the drug without changing the gene expression profile.
None of the genes encoding folate receptors that were analyzed were differentially expressed in cells treated with or without FA.
In conclusion, supplementation with 450 lM FA was not cytotoxic to MCF-7 cells.
However, the addition of FA to anti-cancer drugs must be performed cautiously as the properties of FA might lead to a reduction in drug efficacy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xavier, Muriel Almeida& Oliveira, Marcelo Tempesta de& Baranoski, Adrivanio& Mantovani, Mario Sergio. 2018. Effects of folic acid on the antiproliferative efficiency of doxorubicin, camptothecin and methyl methanesulfonate in MCF-7 cells by mRNA endpoints. Saudi Journal of Biological Sciences،Vol. 25, no. 8, pp.1568-1576.
https://search.emarefa.net/detail/BIM-832858
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xavier, Muriel Almeida…[et al.]. Effects of folic acid on the antiproliferative efficiency of doxorubicin, camptothecin and methyl methanesulfonate in MCF-7 cells by mRNA endpoints. Saudi Journal of Biological Sciences Vol. 25, no. 8 (Dec. 2018), pp.1568-1576.
https://search.emarefa.net/detail/BIM-832858
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xavier, Muriel Almeida& Oliveira, Marcelo Tempesta de& Baranoski, Adrivanio& Mantovani, Mario Sergio. Effects of folic acid on the antiproliferative efficiency of doxorubicin, camptothecin and methyl methanesulfonate in MCF-7 cells by mRNA endpoints. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 8, pp.1568-1576.
https://search.emarefa.net/detail/BIM-832858
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 1575-1576
رقم السجل
BIM-832858
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر