Cytotoxic effects of two extracts from garlic (Allium sativum L.)‎ cultivars on the human squamous carcinoma cell line SCC-15

الملخص EN

Garlic (Allium sativum L., Alliaceae) has acquired a reputation as a therapeutic agent and herbal remedy to prevent and treat several pathologies.

The aim of the present study was to determine the effect of two Allium sativum L.

cultivars, Harnas´ and Morado, on reactive oxygen species (ROS) production, viability and apoptotic process in human squamous carcinoma cell line SCC-15.

The experiments were conducted on SCC-15 cell line exposed to increasing concentrations of garlic extracts of 0.062, 0.125, 0.250, 0.500 and 1.000 mg/mL.

After the experiments, ROS formation, caspase-3 activity and neutral red uptake were measured in the cells, and in a collected medium lactate dehydrogenase (LDH) release was measured.

The Spanish cultivar Morado has demonstrated higher potential to stimulate ROS production in SCC-15 cells after a short time period (6 h) than the Polish cultivar Harnas´.

However, the Polish cultivar Harnas´ manifested more prolonged potential to stimulate ROS production in SCC-15 cells.

Both studied garlic extracts induced cytotoxicity on SCC-15 cell line which was probably ROS-dependent.

We also determined that in SCC-15 cells high concentrations of studied extracts did not cause activation of caspase-3 which suggested caspase-independent or necrotic cell death.