Evaluation of the role of -137G C single nucleotide polymorphism (rs187238) and gene expression levels of the IL-18 in patients with coronary artery disease
المؤلفون المشاركون
Husayni, Fatimah
Mahmazi, Sanaz
Soltanpour, Muhammad Sulayman
Mahmudi, Khalil
Jafari, Ghulam Ali
المصدر
العدد
المجلد 33، العدد 2 (31 مارس/آذار 2018)، ص ص. 118-125، 8ص.
الناشر
المجلس العماني للاختصاصات الطبية
تاريخ النشر
2018-03-31
دولة النشر
سلطنة عمان
عدد الصفحات
8
التخصصات الرئيسية
العلوم الطبية والصيدلة والعلوم الصحية
الطب البشري
الموضوعات
الملخص EN
Objectives: Interleukin-18 (IL-18) is a proinflammatory and proatherogenic cytokine, and its genetic variations may contribute to the development of coronary artery disease (CAD ).
We sought to investigate the role of -137G/C polymorphism and gene expression levels of IL-18 in patients with CAD .
Methods: The study population included 100 patients with angiographically proven CAD and 100 matched controls.
Total RNA and DNA were extracted from leukocytes using appropriate kits.
The genotype of -137G/C polymorphism and gene expression level of IL-18 was determined using allele-specific polymerase chain reaction (PCR ) and real-time (RT)-PCR assay, respectively.
Results: The genotypic and allelic distribution of IL-18 -137G/C polymorphism was not significantly different between the two groups (p > 0.050).
Moreover, the -137G/C polymorphism did not increase the risk of CAD in dominant and recessive genetic models (p > 0.050).
However, subgroup analysis of CAD patients revealed that the IL- 18 -137G/C polymorphism was significantly associated with increased risk of CAD in hypertensive patients (odds ratio (OR) = 7.51; 95% confidence interval (CI): 1.24– 25.17; p = 0.019) and smokers (OR = 4.90; 95% CI: 1.21–19.70; p = 0.031) but not in the diabetic subpopulation (p = 0.261).
The genotype distribution of IL-18 -137G/C genetic polymorphism was significantly different among patients with one, two, and three stenotic vessels (p < 0.050).
The gene expression level of IL-18 was significantly higher in the CAD group than the control group (p < 0.001).
Moreover, the carriers of CC genotype had significantly lower gene expression levels of IL-18 than carriers of GG genotype (p < 0.050).
Conclusions: The -137G/C polymorphism of IL-18 may be associated with the CAD risk in hypertensive and smoker subgroup of CAD patients.
The -137G/C polymorphism seems to play an important role in determining the severity of CAD .
Increased IL-18 gene expression level is a significant risk factor for the development of CAD .
The CC genotype of -137G/C polymorphism is associated with lower IL-18 gene expression levels.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Husayni, Fatimah& Mahmazi, Sanaz& Mahmudi, Khalil& Jafari, Ghulam Ali& Soltanpour, Muhammad Sulayman. 2018. Evaluation of the role of -137G C single nucleotide polymorphism (rs187238) and gene expression levels of the IL-18 in patients with coronary artery disease. Oman Medical Journal،Vol. 33, no. 2, pp.118-125.
https://search.emarefa.net/detail/BIM-835894
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Husayni, Fatimah…[et al.]. Evaluation of the role of -137G C single nucleotide polymorphism (rs187238) and gene expression levels of the IL-18 in patients with coronary artery disease. Oman Medical Journal Vol. 33, no. 2 (Mar. 2018), pp.118-125.
https://search.emarefa.net/detail/BIM-835894
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Husayni, Fatimah& Mahmazi, Sanaz& Mahmudi, Khalil& Jafari, Ghulam Ali& Soltanpour, Muhammad Sulayman. Evaluation of the role of -137G C single nucleotide polymorphism (rs187238) and gene expression levels of the IL-18 in patients with coronary artery disease. Oman Medical Journal. 2018. Vol. 33, no. 2, pp.118-125.
https://search.emarefa.net/detail/BIM-835894
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 124-125
رقم السجل
BIM-835894
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر