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Evaluation of the role of -137G C single nucleotide polymorphism (rs187238) and gene expression levels of the IL-18 in patients with coronary artery disease
المؤلفون المشاركون
Husayni, Fatimah
Mahmazi, Sanaz
Soltanpour, Muhammad Sulayman
Mahmudi, Khalil
Jafari, Ghulam Ali
المصدر
العدد
المجلد 33، العدد 2 (31 مارس/آذار 2018)، ص ص. 118-125، 8ص.
الناشر
المجلس العماني للاختصاصات الطبية
تاريخ النشر
2018-03-31
دولة النشر
سلطنة عمان
عدد الصفحات
8
التخصصات الرئيسية
العلوم الطبية والصيدلة والعلوم الصحية
الطب البشري
الموضوعات
الملخص EN
Objectives: Interleukin-18 (IL-18) is a proinflammatory and proatherogenic cytokine, and its genetic variations may contribute to the development of coronary artery disease (CAD ).
We sought to investigate the role of -137G/C polymorphism and gene expression levels of IL-18 in patients with CAD .
Methods: The study population included 100 patients with angiographically proven CAD and 100 matched controls.
Total RNA and DNA were extracted from leukocytes using appropriate kits.
The genotype of -137G/C polymorphism and gene expression level of IL-18 was determined using allele-specific polymerase chain reaction (PCR ) and real-time (RT)-PCR assay, respectively.
Results: The genotypic and allelic distribution of IL-18 -137G/C polymorphism was not significantly different between the two groups (p > 0.050).
Moreover, the -137G/C polymorphism did not increase the risk of CAD in dominant and recessive genetic models (p > 0.050).
However, subgroup analysis of CAD patients revealed that the IL- 18 -137G/C polymorphism was significantly associated with increased risk of CAD in hypertensive patients (odds ratio (OR) = 7.51; 95% confidence interval (CI): 1.24– 25.17; p = 0.019) and smokers (OR = 4.90; 95% CI: 1.21–19.70; p = 0.031) but not in the diabetic subpopulation (p = 0.261).
The genotype distribution of IL-18 -137G/C genetic polymorphism was significantly different among patients with one, two, and three stenotic vessels (p < 0.050).
The gene expression level of IL-18 was significantly higher in the CAD group than the control group (p < 0.001).
Moreover, the carriers of CC genotype had significantly lower gene expression levels of IL-18 than carriers of GG genotype (p < 0.050).
Conclusions: The -137G/C polymorphism of IL-18 may be associated with the CAD risk in hypertensive and smoker subgroup of CAD patients.
The -137G/C polymorphism seems to play an important role in determining the severity of CAD .
Increased IL-18 gene expression level is a significant risk factor for the development of CAD .
The CC genotype of -137G/C polymorphism is associated with lower IL-18 gene expression levels.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Husayni, Fatimah& Mahmazi, Sanaz& Mahmudi, Khalil& Jafari, Ghulam Ali& Soltanpour, Muhammad Sulayman. 2018. Evaluation of the role of -137G C single nucleotide polymorphism (rs187238) and gene expression levels of the IL-18 in patients with coronary artery disease. Oman Medical Journal،Vol. 33, no. 2, pp.118-125.
https://search.emarefa.net/detail/BIM-835894
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Husayni, Fatimah…[et al.]. Evaluation of the role of -137G C single nucleotide polymorphism (rs187238) and gene expression levels of the IL-18 in patients with coronary artery disease. Oman Medical Journal Vol. 33, no. 2 (Mar. 2018), pp.118-125.
https://search.emarefa.net/detail/BIM-835894
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Husayni, Fatimah& Mahmazi, Sanaz& Mahmudi, Khalil& Jafari, Ghulam Ali& Soltanpour, Muhammad Sulayman. Evaluation of the role of -137G C single nucleotide polymorphism (rs187238) and gene expression levels of the IL-18 in patients with coronary artery disease. Oman Medical Journal. 2018. Vol. 33, no. 2, pp.118-125.
https://search.emarefa.net/detail/BIM-835894
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 124-125
رقم السجل
BIM-835894
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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