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Mesenchymal stem cells improve ischemic stroke injury by anti-inflammatory properties in rat model of middle cerebral artery occlusion
المؤلفون المشاركون
Najati, Majid
Tumah, Abu al-Fadl Azami
Vahidinia, Zaynab
Atlasi, Muhammad Ali
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 20، العدد 1 (31 يناير/كانون الثاني 2018)، ص ص. 1-10، 10ص.
الناشر
تاريخ النشر
2018-01-31
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
10
التخصصات الرئيسية
العلوم الطبية والصيدلة والعلوم الصحية
الملخص EN
Background: Ischemic stroke is a major cause of permanent disability and inflammation has a prominent role in stroke pathology.
Stem cell therapy is a new approach for stroke treatment.
Mesenchymal stem cells (MSCs) are appropriate for this approach due to neuroprotective and immunomodulatory effects.
Objectives: In this experimental study, the neuroprotective effects of mesenchymal stem cells (MSCs) on brain injury after transient middle cerebral artery occlusion (tMCAO) in rats was investigated with emphasis on inflammatory factors.
Methods: Mesenchymal Stem Cells were isolated from bone marrow of rats and expanded by cell culture.
Thirty-six male Wistar rats were randomly selected and divided to 6 groups.
The MCAO model was performed in 4 groups with 24 and 72 hours of reperfusion.
A single infusion of 2106 MSCs was transplanted in one of the 24-hour and 72-hour groups and others received saline.
In the sham groups, surgery was done without MCAO.
Behavioral tests were evaluated and infarct volume was measured by staining of brain sections.
Serumlevels of Interleukin (IL) 1 and Tumornecrosis factor (TNF) were measured by the enzymelinked immunosorbent assay (ELISA).
Relative expression of Interleukin (IL)1 , tumor necrotizing factor (TNF) , and IL6 genes were assessed in penumbra of the ischemic region using real time polymerase chain reaction (PCR).
Results: The study results indicated that total behavioral scores were increased 72 hours after MSC transplantation (14.52.0, P < 0.01).
Moreover, MSCs decreased the infarct volume both 24 hours (18.82 1.58, P < 0.01) and 72 hours (14.4 1.53, P < 0.05) after MCAO.
Serum levels of IL-1 and TNF were increased afterMCAO, yet MSCs transplantation decreased IL-1 (368.3109.5, P < 0.001) and TNF (126.9 38.6, P < 0.01) compared to saline.
Also, relative gene expression of IL1 , TNF , and IL6 was decreased by MSCs transplantation (P < 0.05).
Conclusions: The MSCs had a neuroprotective effect in ischemic stroke via modulation of inflammatory response, and serum levels of IL1 and TNF could be used as markers for evaluating anti-inflammatory effects of MSCs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Najati, Majid& Tumah, Abu al-Fadl Azami& Vahidinia, Zaynab& Atlasi, Muhammad Ali. 2018. Mesenchymal stem cells improve ischemic stroke injury by anti-inflammatory properties in rat model of middle cerebral artery occlusion. Iranian Red Crescent Medical Journal،Vol. 20, no. 1, pp.1-10.
https://search.emarefa.net/detail/BIM-835983
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Najati, Majid…[et al.]. Mesenchymal stem cells improve ischemic stroke injury by anti-inflammatory properties in rat model of middle cerebral artery occlusion. Iranian Red Crescent Medical Journal Vol. 20, no. 1 (Jan. 2018), pp.1-10.
https://search.emarefa.net/detail/BIM-835983
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Najati, Majid& Tumah, Abu al-Fadl Azami& Vahidinia, Zaynab& Atlasi, Muhammad Ali. Mesenchymal stem cells improve ischemic stroke injury by anti-inflammatory properties in rat model of middle cerebral artery occlusion. Iranian Red Crescent Medical Journal. 2018. Vol. 20, no. 1, pp.1-10.
https://search.emarefa.net/detail/BIM-835983
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 9-10
رقم السجل
BIM-835983
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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