Gene expression and polymorphism of interleukin-4 in a sample of Iraqi rheumatoid arthritis patients
العناوين الأخرى
التعبير الجيني و تعدد الأشكال الوراثي للبين ابيضاض-4 في عينة من مرضى التهاب المفاصل الرثوي العراقيين
المؤلفون المشاركون
Mahmud, Asil Shakir
Mayuf, Khudayr Zughayr
al-Qazzaz, Abd al-Karim Abd al-Razzaq Abd al-Wahhab
Adhiah, Ali Husayn
المصدر
العدد
المجلد 15، العدد 2 (30 يونيو/حزيران 2018)، ص ص. 130-137، 8ص.
الناشر
جامعة بغداد كلية العلوم للبنات
تاريخ النشر
2018-06-30
دولة النشر
العراق
عدد الصفحات
8
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الملخص EN
It was aimed to understand the interleukin-4 (IL-4) role in etio-pathogenesis of rheumatoid arthritis (RA).
Two approaches were adopted.
In the first one, a quantitative expression of IL4 gene was assessed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and such findings were correlated with some demographic, clinical and laboratory parameters, which included gender, duration of disease, disease activity score (DAS-28), rheumatoid factors (RFs), C-reactive protein (CRP) and anti-cyclic citrullinated peptide (ACCP) antibodies.
In the second approach, a single nucleotide polymorphism (SNP) of IL4 gene (rs2243250) was inspected by DNA sequencing using specific primers.
Fifty-one Iraqi RA patients (22 males and 29 females) were enrolled in the study.
They were under therapy, which was a single weekly subcutaneous dose of 25 mg of etanercept (Enbrel) for a period of 3-5 years.
The results of gene expression (2-ΔΔCt) revealed an increased expression of IL4 mRNA (Mean ± SEM: 8.247 ± 2.442), especially female patients compared to male patients (11.545 ± 3.928 vs.
3.537 ± 1.530; p = 0.03).
The expression was also subjected to variations that were related to clinical and laboratory findings.
With respect to IL4 gene SNP, allele and genotype frequencies showed no significant differences between RA patients and controls.
In addition, the SNP genotypes had no effect on IL4 gene expression.
In conclusion, an up-regulation of IL4 gene expression was observed in RA patients, and it was more pronounced in female than male patients by approximately four folds, while no association between the IL4 SNP alleles or genotypes and RA was observed.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Adhiah, Ali Husayn& Mahmud, Asil Shakir& al-Qazzaz, Abd al-Karim Abd al-Razzaq Abd al-Wahhab& Mayuf, Khudayr Zughayr. 2018. Gene expression and polymorphism of interleukin-4 in a sample of Iraqi rheumatoid arthritis patients. Baghdad Science Journal،Vol. 15, no. 2, pp.130-137.
https://search.emarefa.net/detail/BIM-837419
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Adhiah, Ali Husayn…[et al.]. Gene expression and polymorphism of interleukin-4 in a sample of Iraqi rheumatoid arthritis patients. Baghdad Science Journal Vol. 15, no. 2 (2018), pp.130-137.
https://search.emarefa.net/detail/BIM-837419
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Adhiah, Ali Husayn& Mahmud, Asil Shakir& al-Qazzaz, Abd al-Karim Abd al-Razzaq Abd al-Wahhab& Mayuf, Khudayr Zughayr. Gene expression and polymorphism of interleukin-4 in a sample of Iraqi rheumatoid arthritis patients. Baghdad Science Journal. 2018. Vol. 15, no. 2, pp.130-137.
https://search.emarefa.net/detail/BIM-837419
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 136-137
رقم السجل
BIM-837419
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر