Studies on paraoxonase-1 isolated from amniotic fluid and its effect against cisplatin-induced hepatotoxicity and cardiotoxicity in rats
العناوين الأخرى
دراسة لإنزيم الباراوكسونيز المعزول من السائل الامنيوني و تأثيراته ضد التسمم الكبدي والقلبي المستحدث بعقار السزبلاتين في الجرذان
المؤلف
المصدر
العدد
المجلد 27، العدد 2 (30 إبريل/نيسان 2018)، ص ص. 42-56، 15ص.
الناشر
تاريخ النشر
2018-04-30
دولة النشر
العراق
عدد الصفحات
15
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الملخص EN
Paraoxonase-1 (PON1) was isolated from the amniotic fluid of normal pregnancies at last week of gestation.
One proteinous band had been isolated by gel filtration sephadex (G-50) from the protein precipitate produced by ammonium sulfate saturation (67%) after dialysis.
The product from (G-50) gave two bands by sephadex (G-100).
It was found that the first peak (Peak A) had higher activity for (PON1).
The apparent molecular weights of the isolated PON1 using gel filtration chromatography and SDS-PAGE was (43873 + 350) and (43682 + 278) Dalton respectively.
The results also showed that the optimum conditions of PON1 was obtained at (80 μg/ml) of protein as a source of the enzyme using (10 mmol/l) of paraoxon as a substrate, Tris-HCl buffer (0.14 mol/l) as a buffer at pH (8.0) and incubation for (7) minutes at (45°C).
Using lineweaver– Burk plot, the values of maximum velocity (Vmax) and Michaelis constant (Km) were (80.0 μmol/ min) and (3.79 mmol/l) respectively.
The protective effect of PON1 against cisplatin-induced hepatotoxicity and cardiotoxicity by intraperitoneal injection of (10mg/kg) cisplatin were evaluated in 35 male albino white rats classified into 5 groups.
The rats were treated with 0.5mg/kg/day or 1mg/kg/day of isolated PON1 injected intraperitonealy for 5 successive days before and 5 successive days after induction of toxicity.
The results showed a significant reduction in the levels of alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase (CK), total bilirubin(TB) and malondialdehyde(MDA) in comparison with the cisplatin treated animals.
It was concluded that (PON1) protects the liver and heart against the toxicity induced by this cytotoxic drug.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Hilali, Luayy Abd Ali. 2018. Studies on paraoxonase-1 isolated from amniotic fluid and its effect against cisplatin-induced hepatotoxicity and cardiotoxicity in rats. Rafidain Journal of Science،Vol. 27, no. 2, pp.42-56.
https://search.emarefa.net/detail/BIM-838939
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Hilali, Luayy Abd Ali. Studies on paraoxonase-1 isolated from amniotic fluid and its effect against cisplatin-induced hepatotoxicity and cardiotoxicity in rats. Rafidain Journal of Science Vol. 27, no. 2 (2018), pp.42-56.
https://search.emarefa.net/detail/BIM-838939
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Hilali, Luayy Abd Ali. Studies on paraoxonase-1 isolated from amniotic fluid and its effect against cisplatin-induced hepatotoxicity and cardiotoxicity in rats. Rafidain Journal of Science. 2018. Vol. 27, no. 2, pp.42-56.
https://search.emarefa.net/detail/BIM-838939
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 53-56
رقم السجل
BIM-838939
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر