Comparison of three DNA extraction methods for detection echinococcus granulosus isolated from sheep and cows
المؤلفون المشاركون
Ahmad, Dunya N.
Jayyad, Abd Allah L.
Muhsin, Salwa Sabr
المصدر
Journal of the Faculty of Medicine Baghdad
العدد
المجلد 60، العدد 1 (31 مارس/آذار 2018)، ص ص. 62-62، 1ص.
الناشر
تاريخ النشر
2018-03-31
دولة النشر
العراق
عدد الصفحات
1
التخصصات الرئيسية
الملخص EN
Background: Hydatosis caused by Echinococcus granulosus dog tap worm is zoonotic infection and economic important and to public health constitutes a major threat in certain regions of Middle East.
There is establishment of preventive and control strategy for characterization of E.granulosus in all endemic area which is essential in all molecular studies, to check the DNA of the parasite.
Objective: Our study aimed to obtain the best from three extractions DNA methods from hydatid cyst protoscolecses isolated from sheep in Al-shawlla abattoir in Baghdad.
Subjects and Methods: Three methods were used to extract DNA samples (boiling, crushing and commercial) DNA samples were performed with electrophoreses on 1.3% agarose.
Regarding DNA for methods were compared by time and facility and cost effectiveness.
Results: The most method were boiling and crushing DNA extraction because of their easy, quickness besides the commercial kit method which had good bands on gel electrophoresis but had most cost effectiveness and time.
Conclusion: The most method were boiling and crushing using for DNA extraction
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ahmad, Dunya N.& Muhsin, Salwa Sabr& Jayyad, Abd Allah L.. 2018. Comparison of three DNA extraction methods for detection echinococcus granulosus isolated from sheep and cows. Journal of the Faculty of Medicine Baghdad،Vol. 60, no. 1, pp.62-62.
https://search.emarefa.net/detail/BIM-839412
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ahmad, Dunya N.…[et al.]. Comparison of three DNA extraction methods for detection echinococcus granulosus isolated from sheep and cows. Journal of the Faculty of Medicine Baghdad Vol. 60, no. 1 (2018), pp.62-62.
https://search.emarefa.net/detail/BIM-839412
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ahmad, Dunya N.& Muhsin, Salwa Sabr& Jayyad, Abd Allah L.. Comparison of three DNA extraction methods for detection echinococcus granulosus isolated from sheep and cows. Journal of the Faculty of Medicine Baghdad. 2018. Vol. 60, no. 1, pp.62-62.
https://search.emarefa.net/detail/BIM-839412
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 64
رقم السجل
BIM-839412
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر