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Detection of biofilm formation and its associated genes in coagulase negative staphylococci isolated from milk of cows affected with subclinical mastitis
العناوين الأخرى
الكشف عن تكوين الأغشية الحيوية و الجينات المصاحبة لها في المكورات العنقودية السلبية لاختبار تلزن البلازما المعزولة من لبن أبقار مصابة بالتهاب الضرع الغير ظاهري
المؤلف
المصدر
Assiut Veterinary Medical Journal
العدد
المجلد 63، العدد 154 (31 يوليو/تموز 2017)، ص ص. 86-92، 7ص.
الناشر
تاريخ النشر
2017-07-31
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Staphylococcus pathogenicity is attributed to the presence of extracellular elements and invasive mechanisms including biofilms formation.
The present study was designed to estimate the frequency of coagulase negative staphylococci (CNS) in cow’s milk affected with subclinical mastitis, determine its ability to form biofilms on polyesterne surface, and for detection of biofilms associated genes (bap, icaA and icaD) using PCR.
Accordingly, a total of 160 milk samples from cows affected with subclinical mastitis based on the results of California Mastitis Test (CMT) were collected from individual quarters of lactating cows.
All collected milk samples were subjected to bacterial isolation procedures following the standard procedures.
The overall prevalence of CNS was 69 (43.12%), S.
chromogenes was the predominate CNS isolates followed by S.
epidermidis.
The phenotypic biofilms formation was tested using microtitre plate technique, 56( 81.15%) strains were tested positive for biofilm formation of them, 53.57% (30/56) were strong, 32.14% (18/ 56) were moderate and 14.28% (8/56) were week biofilms producers.
Among the CNS species, S.
chromogenes was the predominated biofilm producing stains.
Regarding the existence of biofilm associated genes, icaA was detected in 25(36.23%) isolates, icaD was harbored by 33(47.82%) CNS strains and bap gene couldn’t be identified in all of the tested CNS isolates.
icaD was correlated with biofilm formation on polysterne surface of microtitre plate.
In conclusion, this study provides information about the frequency of CNS in bovine subclinical mastitis and its pathogenicity which is necessary to enterprise efficient approaches to control bovine mastitis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Awad, Amal. 2017. Detection of biofilm formation and its associated genes in coagulase negative staphylococci isolated from milk of cows affected with subclinical mastitis. Assiut Veterinary Medical Journal،Vol. 63, no. 154, pp.86-92.
https://search.emarefa.net/detail/BIM-848752
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Awad, Amal. Detection of biofilm formation and its associated genes in coagulase negative staphylococci isolated from milk of cows affected with subclinical mastitis. Assiut Veterinary Medical Journal Vol. 63, no. 154 (Jul. 2017), pp.86-92.
https://search.emarefa.net/detail/BIM-848752
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Awad, Amal. Detection of biofilm formation and its associated genes in coagulase negative staphylococci isolated from milk of cows affected with subclinical mastitis. Assiut Veterinary Medical Journal. 2017. Vol. 63, no. 154, pp.86-92.
https://search.emarefa.net/detail/BIM-848752
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
رقم السجل
BIM-848752
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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