Effects of some physical agents on staphylococcus epidermidis and leishmania tropica : an in vitro study

الملخص EN

This work evaluated the effect of Alpha, Gamma irradiation and Nd:YAG, HeNe laser on Staphylococcus epidermidis and Leishmania tropica in vitro.

The experiment included five replicate of S.

epidermidis , L.

tropica in vitro exposed to effect of Alpha , Gamma irradiation by 241Am isotopes , in two doses ? = 0.31993 MsV (Milli Sievert) for 3 hr, and in dose ?= 1.4157 MsV for 3hr , Americium isotopes give two type of decay Alpha and Gamma Rays.

Effect of Nd:YAG laser in 1000 pulse between each pulse 6 sec, in wavelength 1.06 nm ; also He-Ne laser in 5, 10 , 20 , 30 minute , with wavelength 632.8nm.

The effect of Nd:YAG, He-Ne laser and Alpha, Gamma irradiation on the viability of S.

epidermidis , L.

tropica in vitro was determined by using the specific equation compared with Control and determined by using colorimetric MTT assay respectively , the number of viable cells of S.

epidermidis , L.

tropica was fewer than control (without exposure to laser and irradiation ).

Nd:YAG laser, He-Ne laser and Alpha , Gamma irradiation was efficient to kill S.

epidermidis and L.

tropica