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Molecular and microbiological assessment of stevia (stevia rebaudiana bertoni) leaf extracts for anti-bacterial and anti-mutagenic activities
العناوين الأخرى
التقييم الجزيئي و الميكروبيولوجي لمستخلصات أوراق الأستيفيا (Stevia rebaudiana Bertoni) للأنشطة المضادة للبكتريا و المضادة للطفور
المؤلفون المشاركون
al-Ashtukhi, Muhammad Mamduh Abd al-Tawwab
Taha, Hibah Sayyid Ahmad
المصدر
Zagazig Journal for Agricultural Researches
العدد
المجلد 44، العدد 5 (31 أكتوبر/تشرين الأول 2017)، ص ص. 1683-1692، 10ص.
الناشر
تاريخ النشر
2017-10-31
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The purpose of the study is to assess the anti-bacterial and anti-mutagenic activities of Stevia rebaudiana leaf extracts on some pathogenic bacteria.
Two extracts had been tested which prepared by two different solvents.
Acetone extract was more effective as anti-bacterial than ethyl alcohol extract.
Acetone extract showed high activity against Gram-positive bacteria which recorded (٢٠ mm) while it recorded (١٨ mm) against Gram-negative bacteria.
The study enhanced the antibacterial activity of Stevia rebaudiana leaf extracts using different solvents.
The effectiveness of stevia extract as anti-mutagenic agent on Pseudomonas aeruginosa was also evaluated.
The results of induction of prophage F١١٦ from P.
aeruginosa after treated with different concentrations from each stevia extracts with ascorbic acid as negative control showed that stevia extract has no mutagenic activity on the tested bacteria.
Moreover, the concentrations of ethyl methanesulfonate (EMS) when used as positive control showed a mutagenic effect.
However, ١٠٠ mg/ml of stevia extract had reduced mutagenic activity of EMS.
This finding suggested that stevia leaf extract may be playing a remarkable role in anti-mutagenic response.
Susceptibility of the bacterial strain to exposure to different mutagenic and non-mutagenic agents has been assessed by analysis the variation of protein profile banding pattern (SDS-PAGE).
The comparison between banding pattern when bacteria treated with each of stevia leaf extract, ascorbic acid and EMS, demonstrated that concentration ١٠٠ mg/ml of stevia leaf extract had significant effect to reduce the effect of the EMS as mutagenic agent in bacteria.
Results from this study suggest that natural products like stevia leaf extract could be as an effective agent reducing the effect of the mutagenic agents.
In addition to the antimicrobial properties which can be applied in the medical field that requires further studies and development.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Ashtukhi, Muhammad Mamduh Abd al-Tawwab& Taha, Hibah Sayyid Ahmad. 2017. Molecular and microbiological assessment of stevia (stevia rebaudiana bertoni) leaf extracts for anti-bacterial and anti-mutagenic activities. Zagazig Journal for Agricultural Researches،Vol. 44, no. 5, pp.1683-1692.
https://search.emarefa.net/detail/BIM-854055
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Ashtukhi, Muhammad Mamduh Abd al-Tawwab& Taha, Hibah Sayyid Ahmad. Molecular and microbiological assessment of stevia (stevia rebaudiana bertoni) leaf extracts for anti-bacterial and anti-mutagenic activities. Zagazig Journal for Agricultural Researches Vol. 44, no. 5 (2017), pp.1683-1692.
https://search.emarefa.net/detail/BIM-854055
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Ashtukhi, Muhammad Mamduh Abd al-Tawwab& Taha, Hibah Sayyid Ahmad. Molecular and microbiological assessment of stevia (stevia rebaudiana bertoni) leaf extracts for anti-bacterial and anti-mutagenic activities. Zagazig Journal for Agricultural Researches. 2017. Vol. 44, no. 5, pp.1683-1692.
https://search.emarefa.net/detail/BIM-854055
نوع البيانات
مقالات
لغة النص
الإنجليزية
رقم السجل
BIM-854055
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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