Molecular detection of Anaplasma Marginale in the Egyptian water buffaloes (bubalus bubalis)‎ based on major surface protein 1a

الملخص EN

Anaplasmosis is an infections, non-contagious disease caused by the rickettsial pathogen An-aplasma margin ale.

The organism is globally distributed and infects erythrocytes, resulting in anemia, jaundice, fever, abortions and death.

Once infected, animals remain carriers for life.

In developing countries anaplasmosis is of great economic losses as it is highly endemic.

The present study was designed to determine the prevalence of A.

?narginale in blood samples of buffaloes since they are important reservoir hosts for A.

tnarginale and can serve as a source of infection for tick.

A total of 150 buffalo blood samples was randomly collected from four govemorates and was analyzed using PC'R assay based on mspla.

Anaplasma margmale DNA was detected in 69.3% (104/150) of the sampled buffaloes, and 86.6% (130/150) of collected ticks.

As anaplasmal infection is endemic in Egypt, it was recommended screening herds to de-tect A.

tnarginale even when the signs and symptoms of infection were not visible.