استخلاص إنزيم اللايبيز من بعض أصناف البذور الزيتية المنبتة و دراسة تركيبها الكيمياوي

الملخص EN

The study included purification and extraction lipase EC: 3.1.1.3 from seven varieties of oil seeds before and after germination, those were (soy bean ,rapeseed, sunflower, safflower, pearl millet,yellow corn and castor bean).

The results of activity estimation showed that , the best type of oil seeds are soy bean seeds, they gave the highest specific activity of enzyme before and after germination 5.63 and 9.20 unit/ mg protein respectively.

Lipase improvement was monitored through the germination period of soy bean seeds, it reached the highest activity of lipase after five days of germination ,which was 38.00 unit / ml .The chemical composition of soy bean seeds studied before germination period and through different germination time, higher protein percentage was seen in the germination seeds where it reached its peak at the end of days of germination 41.60 % compared with protein percentage before germination 35.75%.

For the percentage of fat, charbohydrates and ash they decreased in the seed through germination periods, down to 17.75 %, 30.18 % and 5.97% respectively compared with non- germinated 20.79% and 32.15 and 6.26% respectively.