تأثير التوافقات الهرمونية المختلفة في إكثار نبات الداتورة سترامونيوم (Datura stramonium)‎ بالزجاج

الملخص EN

This search was carried out in laboratory of Biotechnology of Medicinal and Aromatic Plants in National Commission for Biotechnology during 2012-2014, for studying the effect of some growth regulators on the length and number of both leaves and branches of micropropagated Datura stramonium plant.

Seeds were surface disinfected by sodium hypochlorite and planted on MS medium, where after, they were transfered onto MS basal medium supplemented with 1mg/L IBA or NAA and (0.5, 1, 1.5, 2) mg/L BAP or K.

After that, plants were transfered onto rooting media containing different concentration of IBA (0, 0.1, 0.5, 1, 2) mg/L.

Results showed that concentration 0.5 of sodium hypochlorite for 10 minutes was the best (28% of germination) on average.

In multiplication experiment, control was exceeded in length of plant (2.04 cm) comparing with all other treatments, while growth regulators increased the number of both leaves and branches, where MS3 and MS4 were exceeded in average of branches number per plant (3.53, 3.43 respectively) and in leaves number per plant (14.64, 12.91 respectively).

The highest percentage of root formation was on the MS medium supported with 2 mg/L IBA (75%).