تجنيس أجنة الأبقار الشامية باستخدام تقانة التفاعل التسلسلي البوليميرازي

الملخص EN

Thirteen viable and post - frozenthawed Shami cows embryos obtained by super-ovulation and artificial insemination technique in order to determine sex of embryos.

Biopsies (1-2 cells) of embryos at morula or blastocyst stage were taken using micromanipulation under inverted microscope.

Blood samples from male and female of Shami cows were also taken as a control.

16 primers were used to amplify DNA fragments using Polymerase Chain Reaction (PCR) to determine possibility of Shami sexing embryos.

Results indicated, for the first time that four out of 16 primers (P1sex, P2sex, P1Aut, P2Aut) were approved to help successfully in showing bands with a size of 141bp distinguishing male embryos of Shami cattle.

It was concluded that aspiration of 1-2 blastomere (s) from embryos at morula or blastocyst stage was enough to determine sex of embryo using PCR and it is recommended that using this technique can be a useful tool in splitting and transferring of desired sex embryos in genetic improvement program.