The effect of rutin on progesterone and estrogen receptor expression in uterine endometrial tissue in the heterotopic transplantation of newborn mouse ovary
المؤلفون المشاركون
Hammadi, Masud
Hadigol, Tayebeh
Sobhani, Aligholi
Nekoonam, Said
Shams, Ali Rida
Farsani, Bahram Islami
Saki, Qasim
Dastoorpoor, Maryam
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 21، العدد 4 (30 إبريل/نيسان 2019)، ص ص. 1-9، 9ص.
الناشر
تاريخ النشر
2019-04-30
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
9
التخصصات الرئيسية
الموضوعات
الملخص EN
Background: Rutin (quercetin-3-rhamnosyl-glucoside), a flavonoid, is derived from plants and has antioxidant properties.
Objectives: This study aimed to evaluate the effect of different concentrations of rutin on mouse ovary heterotopic allotransplantation.
Methods: The present animal experimental study was conducted on 40 female adult Balb/c mice weighing 30±5 g at the Jundishapur University of Medical Sciences, Ahvaz, Iran, during 2016 - 2018.
The mice were divided by permuted block randomization into 8 groups (n = 5): OVX (ovariectomy), as the negative control; normal (positive control); OVX + OVA (ovariectomy and transplantation) (control), treated with 0.5 mL of normal saline; OVX + OVA + 10 mg/kg of rutin; OVX + OVA + 30 mg/kg of rutin; OVX + OVA + 60 mg/kg of rutin; OVX + OVA + 100 mg/kg of rutin; and the autograft.
Groups were treated daily.
Fourteen days after transplantation, ovarian grafts were collected and processed histologically for follicle number counting.
Serum estrogen (E2) and progesterone (P4) levels were evaluated.
Furthermore, the expression of Estrogen Receptor alpha (ERα), Estrogen Receptor beta (ERβ), and Progesterone Receptor (PR) in the uterine endometrial tissue was tested using qRT-PCR and western blotting.
Results: A decrease in the number of mature follicles and increase in the number of atretic follicles (mean ± SD: OVX + OVA + 30 = 19.00± 1.000, OVX + OVA + 60 = 25.00± 5.000, and OVX + OVA + 100 = 23.00 ± 2.646) were observed in all groups treated with rutin in comparison with the control group (mean ± SD: 12.33 ± 2.517) (P value < 0.05).
The level of E2 and P4 (mean ± SD: OVX + OVA + 100 = 6.133 ± 1.026) increased in comparison with the OVX + OVA group (mean ± SD: 0.4667 ± 0.2517) (P value < 0.05).
The protein expression of ERα (mean ± SD: OVX + OVA + 10 = 1.615 ± 0.1701 and OVX + OVA + 30 = 1.744 ± 0.1779) in comparison with the control group (mean ± SD: 0.7089 ± 0.1131), and ERβ (mean ± SD: OVX + OVA + 10 = 0.7747 ± 0.4365, OVX + OVA + 30 = 0.9220 ± 0.1245, OVX + OVA + 60 = 0.7701 ± 0.2150, and OVX + OVA + 100 = 0.6676 ± 0.1547) increased in a dose-dependent manner in all groups treated with rutin in comparison with the OVX + OVA group (mean ± SD: 0.1534 ± 0.06109) (P value < 0.05).
No significant changes in PR were found in groups treated with rutin in comparison with the control group.
Conclusions: The results of the present study indicated that rutin increases E2 and P4 levels in ovarian hetero allograft mice.
Rutin also upregulated the expression of ERα and ERβ but had no significant effect on PR.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hadigol, Tayebeh& Sobhani, Aligholi& Hammadi, Masud& Nekoonam, Said& Shams, Ali Rida& Farsani, Bahram Islami…[et al.]. 2019. The effect of rutin on progesterone and estrogen receptor expression in uterine endometrial tissue in the heterotopic transplantation of newborn mouse ovary. Iranian Red Crescent Medical Journal،Vol. 21, no. 4, pp.1-9.
https://search.emarefa.net/detail/BIM-883776
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hadigol, Tayebeh…[et al.]. The effect of rutin on progesterone and estrogen receptor expression in uterine endometrial tissue in the heterotopic transplantation of newborn mouse ovary. Iranian Red Crescent Medical Journal Vol. 21, no. 4 (Apr. 2019), pp.1-9.
https://search.emarefa.net/detail/BIM-883776
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hadigol, Tayebeh& Sobhani, Aligholi& Hammadi, Masud& Nekoonam, Said& Shams, Ali Rida& Farsani, Bahram Islami…[et al.]. The effect of rutin on progesterone and estrogen receptor expression in uterine endometrial tissue in the heterotopic transplantation of newborn mouse ovary. Iranian Red Crescent Medical Journal. 2019. Vol. 21, no. 4, pp.1-9.
https://search.emarefa.net/detail/BIM-883776
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 8-9
رقم السجل
BIM-883776
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر