Effect of a neoflavonoid (Dalbergin) on T47D breast cancer cell line and mRNA levels of p53, Bcl-2, and STAT3 genes
المؤلفون المشاركون
Valojerdi, Fereshte Mahdizade
Goliaei, Bahram
Nikoofar, Ali Rida
Parivar, Kazim
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 21، العدد 4 (30 إبريل/نيسان 2019)، ص ص. 1-9، 9ص.
الناشر
تاريخ النشر
2019-04-30
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
9
التخصصات الرئيسية
الموضوعات
الملخص EN
Background: Breast cancer is an important cause of death among women.
Prevention of cancer through dietary intervention has recently received increasing interest.
Lately, dietary polyphenols have gained much attention for their health benefits, including anticancer properties.
Dalbergin as a polyphenol is synthesized from a common neoflavene intermediate.
Objectives: This study aimed to examine whether dalbergin can be useful in the chemotherapy of estrogen receptor-positive T47D cell line.
Methods: This experimental study was performed at the Laboratory of Biophysics and Molecular Biology, the Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran, from October 2017 to November 2019.
The doubling time of T47D cells was obtained from the growth curve.
The cytotoxic effect of dalbergin on T47D breast cancer cells was evaluated.
To assess the clonogenic ability, T47D cells were treated with dalbergin for 48 hours and then, the colony assay was performed.
A Real-Time PCR was used to determine the transcription levels of p53, Bcl-2, and STAT3 genes.
Results: The doubling time of T47D cells was 28.02 ± 4.22 hours (P < 0.05).
Dalbergin decreased the viability of the T47D cell line.
The half-maximal inhibitory concentration (IC50) values of dalbergin for T47D cells were found to be 1 µM in 24 hours, 0.001 µM in 48 hours, and 0.00001 µM in 72 hours of treatment (P < 0.05).
In the clonogenic assay, 0.001 µM dalbergin for 48 hours could reduce the surviving fraction of T47D cells (P < 0.05).
Additionally, dalbergin could change the mRNA levels of p53, Bcl-2, and STAT3 genes (P < 0.05).
Conclusions: Our results indicated that dalbergin has some an
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Valojerdi, Fereshte Mahdizade& Goliaei, Bahram& Parivar, Kazim& Nikoofar, Ali Rida. 2019. Effect of a neoflavonoid (Dalbergin) on T47D breast cancer cell line and mRNA levels of p53, Bcl-2, and STAT3 genes. Iranian Red Crescent Medical Journal،Vol. 21, no. 4, pp.1-9.
https://search.emarefa.net/detail/BIM-883777
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Valojerdi, Fereshte Mahdizade…[et al.]. Effect of a neoflavonoid (Dalbergin) on T47D breast cancer cell line and mRNA levels of p53, Bcl-2, and STAT3 genes. Iranian Red Crescent Medical Journal Vol. 21, no. 4 (Apr. 2019), pp.1-9.
https://search.emarefa.net/detail/BIM-883777
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Valojerdi, Fereshte Mahdizade& Goliaei, Bahram& Parivar, Kazim& Nikoofar, Ali Rida. Effect of a neoflavonoid (Dalbergin) on T47D breast cancer cell line and mRNA levels of p53, Bcl-2, and STAT3 genes. Iranian Red Crescent Medical Journal. 2019. Vol. 21, no. 4, pp.1-9.
https://search.emarefa.net/detail/BIM-883777
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 8-9
رقم السجل
BIM-883777
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر