Molecular analysis of V617F mutation in Janus kinase 2 gene of breast cancer patients
المؤلفون المشاركون
Arshad, Muhammad
Karim, Sajjad
Mirza, Zinat
Faruq, Muhammad
Abd al-Jabbar
Mahmud, Asim
Malik, Umran Riyad
Nazir, Quratulain
Yusufi, Qudsiyah
Iqbal, Muhammad Atif
Mahmud, Nasir
Malik, Arif
Asif, Muhammad
Khan, Abd al-Rahman
Rasul, Mahmud
Husayn, Abrar
Zahir, Ahmad
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 26، العدد 6 (30 سبتمبر/أيلول 2019)، ص ص. 1123-1128، 6ص.
الناشر
تاريخ النشر
2019-09-30
دولة النشر
السعودية
عدد الصفحات
6
التخصصات الرئيسية
الموضوعات
الملخص EN
Background: Breast cancer is a multifactorial disease with the highest frequency in females.
Genetic and environmental factors can cause mutation in several genes like tyrosine kinase, JAK2 gene which may initiate cancer.
Molecular analysis of mutations in the JAK2 gene along with determination of environmental, clinical and haematological risk factors associated with breast cancer patients is need of hour to improve patient’s healthcare.
Somatic JAK2 valine-to-phenylalanine (617 codon) mutation is one of the widely prevalent mutations.
Methods: Blood was collected from seventy breast cancer patients after their consent.
The questionnaire included risk factors, age group, locality, number of children, tumor type, family history, time of initial diagnosis, no of cycles/month, water conditions and exposure to radiations.
Molecular analysis were carried out from genomic DNA using Sanger sequencing and allele-specific PCR to check the V617F point mutation.
Results: The breast cancer risk factors includes unfiltered water (68.57%), urban (58.57%), menopause (55.71%), family history of cancer (18.57%), tumor grades (II, 37.14% and III, 35.71%), consanguineous marriages (44.28%) and having more than 3–4 children (45.71%).
Prevalence of breast cancer was higher after the age of 35 and maximum at 35–50.
In allele-specific PCR of 70 patients, 25 patients were wild type (229 bp), 25 patients were with partially deleted gene (200 bp), and 20 patient had shown no or less than 40 bp size fragments.
In Sanger’s sequencing of 70 BC cases, 18% were found to be positive for V617F point mutation, including 6 homozygous (T/T) and 7 heterozygous (G/T) mutations at nucleotide position 1849 in exon 14 of the JAK2 gene.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zahir, Ahmad& Mahmud, Nasir& Malik, Arif& Asif, Muhammad& Khan, Abd al-Rahman& Husayn, Abrar…[et al.]. 2019. Molecular analysis of V617F mutation in Janus kinase 2 gene of breast cancer patients. Saudi Journal of Biological Sciences،Vol. 26, no. 6, pp.1123-1128.
https://search.emarefa.net/detail/BIM-889184
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Malik, Arif…[et al.]. Molecular analysis of V617F mutation in Janus kinase 2 gene of breast cancer patients. Saudi Journal of Biological Sciences Vol. 26, no. 6 (Sep. 2019), pp.1123-1128.
https://search.emarefa.net/detail/BIM-889184
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zahir, Ahmad& Mahmud, Nasir& Malik, Arif& Asif, Muhammad& Khan, Abd al-Rahman& Husayn, Abrar…[et al.]. Molecular analysis of V617F mutation in Janus kinase 2 gene of breast cancer patients. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 6, pp.1123-1128.
https://search.emarefa.net/detail/BIM-889184
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 1128
رقم السجل
BIM-889184
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر