Resveratrol inhibits apoptosis by increase in the proportion of chondrocytes in the S phase of cell cycle in articular cartilage of ACLT plus Mmx rats
المؤلفون المشاركون
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 26، العدد 4 (31 مايو/أيار 2019)، ص ص. 839-844، 6ص.
الناشر
تاريخ النشر
2019-05-31
دولة النشر
السعودية
عدد الصفحات
6
التخصصات الرئيسية
الموضوعات
- الآثار الفسيولوجية
- المواد الكيميائية النباتية
- الغضروف المفصلي
- الخلايا الغضروفية
- موت الخلية المبرمج
- دورة الخلية
الملخص EN
The current study was aimed to investigate the effect of resveratrol on apoptosis inhibition in chondrocytes in ACLT plus Mmx rat model.
TUNEL assay revealed a markedly higher level of apoptotic chondrocytes in the cartilage of untreated ACLT plus Mmx rats.
The percentage of apoptotic chondrocytes was found to be 29.5 and 40.75 %, respectively at 21 and 45 days.
The percentage of apoptotic chondrocytes at 21 and 45 days in resveratrol (5 mg / kg) treated ACLT plus Mmx rats was found to be 13 % and 2 %, respectively.
Real-time PCR analysis revealed that treatment of the ACLT plus Mmx rats with resveratrol for 45 days caused a significant increase in the expression of miR-18a compared to that in untreated rats.
Flow cytometry and BrdUrd incorporation assay revealed that the proportion of chondrocytes in the S phase was increased to 51.4 % in resveratrol treatment group compared to 25.3 % in the untreated ACLT plus Mmx rats.
Western blot analysis showed that treatment of the ACLT plus Mmx rats with resveratrol decreased the expression of ATM protein kinase and GFP protein without any effect on the expression of GFP-!-tubulin in chondrocytes.
In addition, resveratrol treatment also led to reduction in the activity of luciferase in the chondrocytes of ACLT plus Mmx rats.
Resveratrol treatment of the ACLT plus Mmx rats decreases the expression level of ATM protein and checkpoint kinase 2 (CHK2) phosphorylation in chondrocytes.
H2AX and 53BP1 phosphorylation was decreased in ACLT plus Mmx rats on treatment with resveratrol for 45 days.
Immunofluorescence results revealed a markedly lower level of H2AX and 53BP1 nuclear foci in the chondrocytes of ACLT plus Mmx rats treated with resveratrol.
Thus resveratrol treatment of the ACLT plus Mmx rats inhibits chondrocyte apoptosis and increases proportion of cells in the S phase of cell cycle which may be through the increase in expression of miR18a.
A significant relation appears between resveratrol and miR-18a expression in the chondrocytes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Yu& Bai, Lunhao. 2019. Resveratrol inhibits apoptosis by increase in the proportion of chondrocytes in the S phase of cell cycle in articular cartilage of ACLT plus Mmx rats. Saudi Journal of Biological Sciences،Vol. 26, no. 4, pp.839-844.
https://search.emarefa.net/detail/BIM-891169
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Yu& Bai, Lunhao. Resveratrol inhibits apoptosis by increase in the proportion of chondrocytes in the S phase of cell cycle in articular cartilage of ACLT plus Mmx rats. Saudi Journal of Biological Sciences Vol. 26, no. 4 (May. 2019), pp.839-844.
https://search.emarefa.net/detail/BIM-891169
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Yu& Bai, Lunhao. Resveratrol inhibits apoptosis by increase in the proportion of chondrocytes in the S phase of cell cycle in articular cartilage of ACLT plus Mmx rats. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 4, pp.839-844.
https://search.emarefa.net/detail/BIM-891169
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 844
رقم السجل
BIM-891169
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر