Isolation of Escherichia coli from diarrhea and test their pathogensity and susceptibility pattern for antibiotic

الملخص EN

This study was designed to isolate Escherichia coli from diarrhea in children, and to test susceptibility pattern for antibiotic and pathogenesis of Escherichia coli.

150 samples of stool gathered from patients children in different region of Dhi-Qar city in south of Iraq for the period January 2015 to June 2015.

the isolate was microscopically and biochemically examined and diagnosed by Api 20E kit Results of the bacterial growth showed that 111 samples were positive bacterial growth with percentage (74%), 84 isolate belong to Escherichia coli with percentage 75.6%.

The sensitivity of isolates was examined for antibiotics, the isolates showed a height resistance for Ampicillin, while the others showed highest sensitivity for Erythromycin, and Co – trimoxazol.

The study showed the ability of Escherichia coli to elicit an inflammatory response in mouse intestine after experimental infection that induced by orally dosing with Escherichia coli.