Genetic diversity among horse lines in Erbil Region using RAPD markers
المؤلفون المشاركون
Said, Chiman Hamid
Abd al-Razzaq, Huriyyah S.
Qadir, Nazhad H.
المصدر
ZANCO Journal of Pure and Applied Sciences
العدد
المجلد 31، العدد 3 (30 يونيو/حزيران 2019)، ص ص. 39-44، 6ص.
الناشر
جامعة صلاح الدين قسم النشر العلمي
تاريخ النشر
2019-06-30
دولة النشر
العراق
عدد الصفحات
6
التخصصات الرئيسية
الموضوعات
الملخص EN
Genetic diversity is the basis for present day diversified living systems and future genetic improvement needs.
Within the framework of breed conservation, genetic characterization is important in guarding breeds and is a prerequisite for managing genetic resources.
The objective of this study was to use the RAPD technique to evaluate genetic diversity and relatedness within and among four horse line (white, Gray, Brown, Black).
To our knowledge there is currently no information about RAPD genetic markers that detect genetic polymorphism in Erbil/Iraqi horse breeds.
Information from this work provides basic genetic knowledge that is critical for conservation and breeding programs.
Random amplification of polymorphic DNA (RAPD-PCR) was done by using 10 primers from GenScript USA company.
A total of (6) Primers out of the (10) Primers gave results to find a complementary DNA Genomic sites, (OPQ-05, OPQ-06, OPQ-08, OPQ-09, OPQ-10, OPQ-12).
These primers amplified on average 7 to 53 bands of sizes varying from 100bp to 1500bp.
A total of 150 diagnostic bands were scored within RAPD profiles amplified by these 6 primers.
Among 150 scorable bands 28 (18.67%) were recognized as polymorphic.
UPGMA dendrogram based on Nei’s genetic distance grouped the investigated horse line genotypes into two clusters.
The first cluster includes( white, Brown, Black) whereas the second cluster include Gray which appeared to be most distant from the other lines.
In conclusion, these results indicated the effectiveness of RAPD in detecting polymorphism between horse lines and their applicability in lines studies and establishing genetic relationships among the horse lines.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abd al-Razzaq, Huriyyah S.& Said, Chiman Hamid& Qadir, Nazhad H.. 2019. Genetic diversity among horse lines in Erbil Region using RAPD markers. ZANCO Journal of Pure and Applied Sciences،Vol. 31, no. 3, pp.39-44.
https://search.emarefa.net/detail/BIM-892366
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Abd al-Razzaq, Huriyyah S.…[et al.]. Genetic diversity among horse lines in Erbil Region using RAPD markers. ZANCO Journal of Pure and Applied Sciences Vol. 31, no. 3 (2019), pp.39-44.
https://search.emarefa.net/detail/BIM-892366
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abd al-Razzaq, Huriyyah S.& Said, Chiman Hamid& Qadir, Nazhad H.. Genetic diversity among horse lines in Erbil Region using RAPD markers. ZANCO Journal of Pure and Applied Sciences. 2019. Vol. 31, no. 3, pp.39-44.
https://search.emarefa.net/detail/BIM-892366
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 43-44
رقم السجل
BIM-892366
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر