Molecular characterization of newcastle disease virus, quail strain, using one step rt-pcr

الملخص EN

Quails have been a tremendous potential for village and backyard production as well as an important laboratory animal.

Newcastle disease (ND) has become a threat to poultry industry and transmitted to different species of birds including quails.

Newcastle disease is caused by avian paramyxovirus serotype-I, an enveloped, single stranded RNA virus with a 15 Kb genome of negative polarity which codes for six proteins.

In this study a reverse transcriptase- PCR assay (RT-PCR) had been used for detection and genetic characterization of the ND virus from quails in Egypt.

Nucleotide sequencing analysis of selected cases from quails showed that the similarity of the detected virus was 100 % to different published poultry strains.

The new isolate was genetically characterized as mesogenic / velogenic strain.