Selection of male goat embryos in vitro by using swim-up of epididymal spermatozoa
العناوين الأخرى
اختيار الأجنة الذكرية للماعز مختبريا باستعمال طريقة السباحة للأعلى لنطف البربخ
المؤلفون المشاركون
al-Dulaymi, Sura Shakir Hammud
al-Tamimi, Ihsan Hammudi Said
المصدر
Iraqi Journal of Veterinary Medicine
العدد
المجلد 42، العدد 1 (30 يونيو/حزيران 2018)، ص ص. 79-86، 8ص.
الناشر
تاريخ النشر
2018-06-30
دولة النشر
العراق
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The main objectives of this study is the separation of X from Y bearing epididymal spermatozoa of local buck by swim-up, and the use of this spermatozoa for in vitro fertilization to determine the percentage of produced male and female embryos.
The sex of produced embryo was identified by polymerase chain reaction.
Testis of the local buck were obtained from Al-Shu'alah abattoir and the epididymal spermatozoa were harvested from the cauda by and submitted to in vitro maturation prior to separation of X from Y bearing spermatozoa and prior to their use for in vitro fertilization.
For the separation of epididymal spermatozoa, swim-up technique was used with centrifugation at 200×g or 300×g.
The centrifugation at 200×g showed that 41.84±1.39 % of spermatozoa were detected in the supernatant while the precipitate contained 50.69±0.71 and the mean of the sperm lost was 7.65±0.93.
After centrifugation, spermatozoa in the supernatant were used for in vitro fertilization of matured oocytes.
The sex of in vitro produced goat embryos was determined by polymerase chain reaction using specific primers to detect of SRY gene.
The percentage of total goat embryos obtained after in vitro fertilization by sperms selected using swim-up at centrifugation force of 200×g recorded 79.66 % male embryos while female embryos recorded only 20.33 %.
At the end, the results showed the ability of selection male embryos in caprine by application of swim-up technique on epididymal spermatozoa with centrifugation at 200×g.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Dulaymi, Sura Shakir Hammud& al-Tamimi, Ihsan Hammudi Said. 2018. Selection of male goat embryos in vitro by using swim-up of epididymal spermatozoa. Iraqi Journal of Veterinary Medicine،Vol. 42, no. 1, pp.79-86.
https://search.emarefa.net/detail/BIM-897315
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Dulaymi, Sura Shakir Hammud& al-Tamimi, Ihsan Hammudi Said. Selection of male goat embryos in vitro by using swim-up of epididymal spermatozoa. Iraqi Journal of Veterinary Medicine Vol. 42, no. 1 (2018), pp.79-86.
https://search.emarefa.net/detail/BIM-897315
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Dulaymi, Sura Shakir Hammud& al-Tamimi, Ihsan Hammudi Said. Selection of male goat embryos in vitro by using swim-up of epididymal spermatozoa. Iraqi Journal of Veterinary Medicine. 2018. Vol. 42, no. 1, pp.79-86.
https://search.emarefa.net/detail/BIM-897315
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 84-85
رقم السجل
BIM-897315
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر