Molecular prediction for the common causative agents of infectious conjunctivitis among AL-Najaf AL-Ashraf governorate patients in spring and summer
المؤلفون المشاركون
al-Shammari, Ahmad Ali Nizar
Nimah, Ghayth al-Din
al-Shami, Zaynah Muhammad Abd al-Husayn
المصدر
al-Kufa University Journal for Biology
العدد
المجلد 10، العدد 2 (31 أغسطس/آب 2018)، ص ص. 59-66، 8ص.
الناشر
جامعة الكوفة كلية العلوم قسم علوم الحياة
تاريخ النشر
2018-08-31
دولة النشر
العراق
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Aim of the study: is to predict the most common causative agents of infectious conjunctivitis among AL-Najaf AL-Ashraf governorate during spring and summer seasons.
Methods : Multiplex PCR was used to amplify viral and bacterial genes related to pathogens suspected of causing infectious conjunctivitis, the samples were collected from patients using cotton eye swabs.
Results: A total of 36 (10.29%) patients were chosen from 350 patients who attended private ophthalmic clinic in AL-Najaf AL-Ashraf governorate during spring and summer seasons.
Two Cotton swabs were taken from each patient and kept frozen until the day of DNA extraction.
Multiplex PCR was used to amplify genes specific for Adenovirus, Herpes Simplex Virus-1 and Herpes Simplex Virus-2, Cytomegalovirus, Varicella Zoster Virus and Chlamydia trachomitis plasmid.
The multiplex PCR results found that only ten (27.77%) samples were positive for mixed infection of Adenovirus (140bp), HSV-1and HSV DNA polymerase (208, 211 bp) and only one (2.77%) sample was positive for HSV-1mixed with HSV DNA polymerase .
Conclusion: Laboratory identification of infectious agents causing conjunctivitis is crucial for confirming the clinical diagnosis, although some signs may be diagnostic for certain types of eye disorders because infectious and allergic conjunctivitis signs and symptoms may overlap.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Shammari, Ahmad Ali Nizar& Nimah, Ghayth al-Din& al-Shami, Zaynah Muhammad Abd al-Husayn. 2018. Molecular prediction for the common causative agents of infectious conjunctivitis among AL-Najaf AL-Ashraf governorate patients in spring and summer. al-Kufa University Journal for Biology،Vol. 10, no. 2, pp.59-66.
https://search.emarefa.net/detail/BIM-898972
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Shammari, Ahmad Ali Nizar…[et al.]. Molecular prediction for the common causative agents of infectious conjunctivitis among AL-Najaf AL-Ashraf governorate patients in spring and summer. al-Kufa University Journal for Biology Vol. 10, no. 2 (2018), pp.59-66.
https://search.emarefa.net/detail/BIM-898972
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Shammari, Ahmad Ali Nizar& Nimah, Ghayth al-Din& al-Shami, Zaynah Muhammad Abd al-Husayn. Molecular prediction for the common causative agents of infectious conjunctivitis among AL-Najaf AL-Ashraf governorate patients in spring and summer. al-Kufa University Journal for Biology. 2018. Vol. 10, no. 2, pp.59-66.
https://search.emarefa.net/detail/BIM-898972
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 65-66
رقم السجل
BIM-898972
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر