Isolation and identification of bacterial wound infection isolates and their antibiotic susceptibility pattern

الملخص EN

Wound Infections are continuous to be a major complication with significant increase in costs, morbidity and potential mortality.

This study was aim to screening the bacteriological observation on wound infection in both inpatients and outpatients, the effective antibiotic and serotyping for the prevalent bacteria.

The study was carried out on two hundred patients of different age groups to isolate the causative organisms encountered in wound infection and their antibiotics.

The percentage of wound infection among outpatients was found to be 84%, similarly the percentage of wound contamination inside the hospital (Medical City Teaching Hospital) also was found to be 84%.

The relation between sex and wound infection was studied, no effect of sex on the rate of infection was found.A total of 152 bacterial isolates were predominant Staphylococcus aureus (76, 50%), followed by Escherichia coli (36, 23.69%), Beta-haemolytic streptococci(13,8.55%),Klebsiella aerogenes (8,5.26%), Pseudomonas aeruginosa(7,4.6%), Proteus spp.(7,4.6%) and Streptococcus faecalis(5,3.29%).S.aureuswas the most common among outpatients(57%), bacteriophagetyping of this bacteria was made on the total number (76 isolates )from both inpatient and outpatients, thirty-two strains found to be typable, while the remaining (44 strains) were not typable; no predominance of a particular phage-group was observed.

E.

coli was the common bacteria among inpatients (28%), the rate of fecal carriage were 100%.

The antibiotics of both, fecal and wound of the same patient were similar.

Beta-haemolytic streptococci isolated at a rate of 6.5% and group A was the most prevalent group in wound infection.

Regarding the antibiotic susceptibility, superiority of resistance to many antibiotics in treating Pseudomonas aeruginosa, Proteus spp., E.coli, S.

aureus and S.

faecalis was observed.

Most wound Infections were polymicrobial and predominant isolate was S.

aureus.

Isolates showed high level of resistance to antibiotics, which invites us to panic and anxiety.