Detection of Thiopurine S-Methyltransferase (TPMT) polymorphisms TPMT * 3A, TPMT * 3B and TPMT * 3C in children with Acute lymphoblastic leukemia
العناوين الأخرى
الكشف عن وجود طفرات الانزيم ثايوبيورين مثايل ترانسفيريز (TPMT*3A و TPMT*3B و TPMT*3C) في الأطفال العراقيين المصابين بسرطان الدم اللمفاوي الحاد
المؤلفون المشاركون
Ahmad, Shayma Jamal
Muhammad, Nawar Samir
Rashid, Manal Kamal
Ghali, Hasanayn Habib
المصدر
Journal of the Faculty of Medicine Baghdad
العدد
المجلد 60، العدد 3 (30 سبتمبر/أيلول 2018)، ص ص. 166-171، 6ص.
الناشر
تاريخ النشر
2018-09-30
دولة النشر
العراق
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Background : Thiopurines are essential medications in Acute Lymphoblastic Leukemia (ALL) treatment protocols as anti-cancer agents since long time; however, their use might result in unexpected toxicities in ALL children due to the low thiopurine S-methyltransferase (TPMT) activity, a major enzyme involved in 6- mercaptopurine metabolism, which strongly correlates to the genetic polymorphism of the TPMT gene in those patients.
Objective : To identify the most common TPMT polymorphisms in children with ALL and its frequencies.
Methods : A cross sectional study enrolling eighty-one ALL children receiving mercaptopurine drug during their maintenance course of treatment according to UKALL – 2011 protocol, were enrolled in this study.
After DNA extraction from whole blood TPMT genetic polymorphisms were detected by allele-specific multiplex-PCR analysis.
Results : A total of 51 children with allele frequencies of (62.96 %) were homozygous for the wildtype allele TPMT*1, 30 children with allelic frequency of (37.03 %) were heterozygous for one of the two mutant alleles (TPMT*3A or TPMT*3C) with allele frequencies of 29.62 % and 7.4 % respectively, while no result was found homozygous for two mutant alleles or TPMT*3B allele.
Conclusions : This is the first study in Iraq to identify the genetic polymorphism of TPMT in a group of ALL children being treated for ALL.
The study revealed the presence of TPMT*3A and TPMT*3C genetic polymorphisms among the study sample, no TPMT*3B was identified in the study sample.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Muhammad, Nawar Samir& Rashid, Manal Kamal& Ghali, Hasanayn Habib& Ahmad, Shayma Jamal. 2018. Detection of Thiopurine S-Methyltransferase (TPMT) polymorphisms TPMT * 3A, TPMT * 3B and TPMT * 3C in children with Acute lymphoblastic leukemia. Journal of the Faculty of Medicine Baghdad،Vol. 60, no. 3, pp.166-171.
https://search.emarefa.net/detail/BIM-899551
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Muhammad, Nawar Samir…[et al.]. Detection of Thiopurine S-Methyltransferase (TPMT) polymorphisms TPMT * 3A, TPMT * 3B and TPMT * 3C in children with Acute lymphoblastic leukemia. Journal of the Faculty of Medicine Baghdad Vol. 60, no. 3 (2018), pp.166-171.
https://search.emarefa.net/detail/BIM-899551
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Muhammad, Nawar Samir& Rashid, Manal Kamal& Ghali, Hasanayn Habib& Ahmad, Shayma Jamal. Detection of Thiopurine S-Methyltransferase (TPMT) polymorphisms TPMT * 3A, TPMT * 3B and TPMT * 3C in children with Acute lymphoblastic leukemia. Journal of the Faculty of Medicine Baghdad. 2018. Vol. 60, no. 3, pp.166-171.
https://search.emarefa.net/detail/BIM-899551
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 169-170
رقم السجل
BIM-899551
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر