Plasmid transformation and curing of nalidixic acid gene in staphylococcus aureus isolated from buffaloes mastitis and workerʼs hands
العناوين الأخرى
التحول و التحييد البلازميدي لجين Nalidixic Acid في المكورات العنقودية الذهبية المعزولة من التهاب الضرع في الجاموس و أيدي العاملين
المؤلفون المشاركون
Khalil, Dua Abd al-Razzaq
Khudayr, Bassam Yasin
al-Salam, Rasha Mundhir Uthman
المصدر
Iraqi Journal of Veterinary Sciences
العدد
المجلد 32، العدد 2 (31 ديسمبر/كانون الأول 2018)، ص ص. 167-174، 8ص.
الناشر
جامعة الموصل كلية الطب البيطري
تاريخ النشر
2018-12-31
دولة النشر
العراق
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The present study was designed to detect resistant site of nalidixic acid through transformation and plasmid curing of S.
aureus strains isolated from buffalo milk with subclinical mastitis and workers’ hands.
A total of 37 S.
aureus isolates including 17 isolates recovered from buffalo milk infected with subclinical mastitis, in addition to 20 isolates recovered from workers’ hands.
All 37 isolates were investigated by detection of the 23S rRNA gene and various other species specific genes including coa, nuc and clfA.
The antibiotic resistance of S.
aureus isolates was performed by the discs diffusion method using 19 antibiotics.
Plasmid transformation method was carried out by transferring the plasmid isolated from S.
aureus into competent Escherichia coli HB 101 in order to detection the resistant site of nalidixic acid.
Plasmid curing was accomplished by preparing different concentrations of nalidixic acid (100, 150, 200, 250 and 300 μg/ml) and cultured transformed E.coli on LB agar supported with each of the aforementioned concentrations.
The molecular results showed that six isolates (five isolates from milk samples and one from workers’ hands) were identified as S.
aureus by coa, nuc, and clfA species specific primers.
The six S.
aureus isolates were found to be resistant to at least 5 antibiotics which included the nalidixic acid.
The results of plasmid transformation revealed that E.
coli was able to grow on LB agar supported with 100μg/ml, 150 μg/ml, 200 μg/ml and 250 μg/ml of nalidixic acid and failed to grow on 300 μg/ml concentration.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Khalil, Dua Abd al-Razzaq& al-Salam, Rasha Mundhir Uthman& Khudayr, Bassam Yasin. 2018. Plasmid transformation and curing of nalidixic acid gene in staphylococcus aureus isolated from buffaloes mastitis and workerʼs hands. Iraqi Journal of Veterinary Sciences،Vol. 32, no. 2, pp.167-174.
https://search.emarefa.net/detail/BIM-900413
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Khalil, Dua Abd al-Razzaq…[et al.]. Plasmid transformation and curing of nalidixic acid gene in staphylococcus aureus isolated from buffaloes mastitis and workerʼs hands. Iraqi Journal of Veterinary Sciences Vol. 32, no. 2 (2018), pp.167-174.
https://search.emarefa.net/detail/BIM-900413
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Khalil, Dua Abd al-Razzaq& al-Salam, Rasha Mundhir Uthman& Khudayr, Bassam Yasin. Plasmid transformation and curing of nalidixic acid gene in staphylococcus aureus isolated from buffaloes mastitis and workerʼs hands. Iraqi Journal of Veterinary Sciences. 2018. Vol. 32, no. 2, pp.167-174.
https://search.emarefa.net/detail/BIM-900413
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Text in English ; abstracts in English and Arabic.
رقم السجل
BIM-900413
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر