Circulating miRNAs as biomarkers of Type II diabetes mellitus patients in Gaza Strip

العناوين الأخرى

جزيئات الحامض النووي الريبوزي الصغيرة كعلامة حيوية لمرضى السكري من النوع الثاني في قطاع غزة

مقدم أطروحة جامعية

Qaddurah, Nidal Muhammad Awad Ibrahim

مشرف أطروحة جامعية

al-Uwayni, Saib Husayn
Ayish, Basim Muhammad

الجامعة

الجامعة الإسلامية

الكلية

كلية العلوم

القسم الأكاديمي

قسم العلوم الحياتية

دولة الجامعة

فلسطين (قطاع غزة)

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2018

الملخص الإنجليزي

Background and Problem.

Type II Diabetes mellitus (T2DM) is one of the metabolic syndrome and recognized as one of the most serious health problems worldwide.

Compared with people without diabetes, individuals with T2DM have a significantly higher risk of cardiovascular morbidity and mortality.

Heart attack and stroke are the major causes of early death in people with diabetes.

Prognostic biomarkers including Low density lipoprotein (LDLC), cholesterol (TC), Triglycerides (TG) and high density lipoprotein (HDL-C) and high sensitive C-reactive protein (hsCRP) are widely used to predict cardiovascular diseases (CVDs) in T2DM patients in different population.

Recent studies have suggested that microRNAs (miRNAs) play a critical role in the pathogenesis of diabetes and its related cardiovascular complications.

Objectives and aims.

This study is a case-control study aimed to investigate the significance of 8 miRNAs (hsa-miR-29a, hsa-miR-375, hsa-miR-103a-3p, hsa-miR-124-3p, hsa-miR-155-5p and hsa-miR-146a-5p, miR-126 and miR-222) in the prediction of cardiovascular complications among 86 T2DM patients diagnosed with and without cardiovascular diseases (CVDs) and 30 healthy individuals.

Methodology.

Total RNA, including miRNA was isolated from serum samples using the ISOLATE II Biofluids.

hsCRP was measured using immunoturbidimetric technique.

TC, TG and HDL-C were measured using colorimetric and kinetic methods and LDL-C was calculated by empirical relationship of Friedewald.

Fasting blood sugar (FBS), hemoglobinA1c (HBA1c), Lactate dehydrogenase (LDH), Creatine phosphokinase (CPK), Aspartate aminotransferase (AST), were measured using enzymatic methods.

Results.

The levels of hsCRP, FBS, HbA1c, TC, TG, LDL, LDH, CPK, were significantly increased among T2DM patients compared with the normal control (P≤0.05).

Importantly, 68.6%, 87.2% and 48.8% of the cases showed high level of FBS, HbA1c and hsCRP respectively; 35% of cases had high levels of cholesterol > 240 mg/dl while 68% of cases have high level of TG >150mg/dl; 39.6% of cases have high level of LDL-c >130mg/dl and 34.8% of cases have low level of HDL-c <40mg/dl.

The expression levels of all tested miRNAs except of miR-375 were significantly decreased in patients groups in comparison with healthy persons.

Importantly, among all tested miRNAs, hsa-miR-126-5p showed the highest diagnostic accuracy as revealed by receiver operating characteristic (ROC) curve analysis and can relatively discriminate between patients groups and control.

Conclusions.

Taken together serum miRNAs are differentially expressed between T2DM, T2DM with cardiovascular disorders and healthy persons and could be considered as a candidate’s biomarkers to predict these disease which warrants further evaluation and optimization.

التخصصات الرئيسية

الأحياء
الطب البشري

الموضوعات

• مرض السكري
• قطاع غزة
• الحمض النووي الريبوزي

عدد الصفحات

125

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-900926