Human carbonic anhydrase : purification and characterization study in thalassemia major patients compared to healthy subjects
المؤلفون المشاركون
Husayn, Salwa Salih
Zainal, Isra G.
المصدر
العدد
المجلد 15، العدد 4 (31 ديسمبر/كانون الأول 2018)، ص ص. 349-356، 8ص.
الناشر
تاريخ النشر
2018-12-31
دولة النشر
العراق
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Background: Carbonic anhydrase (CA) catalyzes the reversible reaction of converting carbon dioxide to bicarbonate.
Objective: This study was aimed to isolate and purify human erythrocytes CA and study its physicochemical properties of the enzyme reaction for ß-thalassemia major patients.
Materials and Methods: The blood samples included 61 samples of blood (31 males and 30 females) from ßhalassemia patients visited Azadi Hospital/Kirkuk city.
Healthy individuals as control group included 40 participants.
The separated fractions were obtained using four steps: extraction by ethanol and chloroform, ammonium sulfate precipitation, dialysis, and gel filtration chromatography; finally, the CA was analyzed by polyacrylamide gel electrophoresis.
Results: The CA activity showed significant (P ≤ 0.05) decrease, total protein showed nonsignificant (P ≥ 0.05) increase, and specific activity significantly (P ≤ 0.05) increased in patients group compared to healthy individuals.
CA was partially purified with a factor of 22.5 and 18 by extraction with ethanol and chloroform and 1.5,1.4 for Fraction I and 1,2 for Fraction II using gel filtration chromatography.
The optimum conditions for the CA reaction in patients group were enzyme concentration (6 µl), substrate concentration (6 Mm), pH = 7.4, and temperature 37°C.
The electrophoresis study indicated that the bands of CA in patients group showed bands with less intensity than the bands in healthy individuals.
Conclusion: The best method to purify CA from human erythrocytes with high recovery and fold of purification was ethanol–chloroform extraction.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Husayn, Salwa Salih& Zainal, Isra G.. 2018. Human carbonic anhydrase : purification and characterization study in thalassemia major patients compared to healthy subjects. Medical Journal of Babylon،Vol. 15, no. 4, pp.349-356.
https://search.emarefa.net/detail/BIM-902659
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Husayn, Salwa Salih& Zainal, Isra G.. Human carbonic anhydrase : purification and characterization study in thalassemia major patients compared to healthy subjects. Medical Journal of Babylon Vol. 15, no. 4 (Oct. / Dec. 2018), pp.349-356.
https://search.emarefa.net/detail/BIM-902659
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Husayn, Salwa Salih& Zainal, Isra G.. Human carbonic anhydrase : purification and characterization study in thalassemia major patients compared to healthy subjects. Medical Journal of Babylon. 2018. Vol. 15, no. 4, pp.349-356.
https://search.emarefa.net/detail/BIM-902659
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 355-356
رقم السجل
BIM-902659
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر