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Human carbonic anhydrase : purification and characterization study in thalassemia major patients compared to healthy subjects
المؤلفون المشاركون
Husayn, Salwa Salih
Zainal, Isra G.
المصدر
العدد
المجلد 15، العدد 4 (31 ديسمبر/كانون الأول 2018)، ص ص. 349-356، 8ص.
الناشر
تاريخ النشر
2018-12-31
دولة النشر
العراق
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Background: Carbonic anhydrase (CA) catalyzes the reversible reaction of converting carbon dioxide to bicarbonate.
Objective: This study was aimed to isolate and purify human erythrocytes CA and study its physicochemical properties of the enzyme reaction for ß-thalassemia major patients.
Materials and Methods: The blood samples included 61 samples of blood (31 males and 30 females) from ßhalassemia patients visited Azadi Hospital/Kirkuk city.
Healthy individuals as control group included 40 participants.
The separated fractions were obtained using four steps: extraction by ethanol and chloroform, ammonium sulfate precipitation, dialysis, and gel filtration chromatography; finally, the CA was analyzed by polyacrylamide gel electrophoresis.
Results: The CA activity showed significant (P ≤ 0.05) decrease, total protein showed nonsignificant (P ≥ 0.05) increase, and specific activity significantly (P ≤ 0.05) increased in patients group compared to healthy individuals.
CA was partially purified with a factor of 22.5 and 18 by extraction with ethanol and chloroform and 1.5,1.4 for Fraction I and 1,2 for Fraction II using gel filtration chromatography.
The optimum conditions for the CA reaction in patients group were enzyme concentration (6 µl), substrate concentration (6 Mm), pH = 7.4, and temperature 37°C.
The electrophoresis study indicated that the bands of CA in patients group showed bands with less intensity than the bands in healthy individuals.
Conclusion: The best method to purify CA from human erythrocytes with high recovery and fold of purification was ethanol–chloroform extraction.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Husayn, Salwa Salih& Zainal, Isra G.. 2018. Human carbonic anhydrase : purification and characterization study in thalassemia major patients compared to healthy subjects. Medical Journal of Babylon،Vol. 15, no. 4, pp.349-356.
https://search.emarefa.net/detail/BIM-902659
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Husayn, Salwa Salih& Zainal, Isra G.. Human carbonic anhydrase : purification and characterization study in thalassemia major patients compared to healthy subjects. Medical Journal of Babylon Vol. 15, no. 4 (Oct. / Dec. 2018), pp.349-356.
https://search.emarefa.net/detail/BIM-902659
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Husayn, Salwa Salih& Zainal, Isra G.. Human carbonic anhydrase : purification and characterization study in thalassemia major patients compared to healthy subjects. Medical Journal of Babylon. 2018. Vol. 15, no. 4, pp.349-356.
https://search.emarefa.net/detail/BIM-902659
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 355-356
رقم السجل
BIM-902659
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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