Prokaryotic expression and purification of recombinant human leukemia inhibitory factor : analysis of the ability to maintain pluripotency in embryonic stem cells
المؤلفون المشاركون
Miryounesi, Muhammad
Tahiri, Behrouz
Hashemitabar, Mahmud
Mutivasili, Ilahi
Mudarrissi, Muhammad Husayn
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 20، العدد 8 (31 أغسطس/آب 2018)، ص ص. 1-6، 6ص.
الناشر
تاريخ النشر
2018-08-31
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Background : Leukemia Inhibitory Factor (LIF) is largely used in stem cell researches for the maintenance of Embryonic Stem Cells (ESCs) in a pluripotent state.
However, the relatively high costs of LIF is a potential limit of such researches.
Objectives : The aim of this study was prokaryotic expression and purification of the recombinant hexahistidine-tagged human LIF (His6-hLIF) fusion protein and assessment of its ability to maintain a pluripotent or undifferentiated state of ESCs.
Methods : Encoding the DNA sequence of mature hLIF was codon optimized for expression in Escherichia coli and chemically synthesized and cloned in the expression vector pET- 28a (+).
Immobilized Metal Affinity Chromatography (IMAC) was performed to purify the recombinant His6-hLIF.
Then, His6-hLIF was tested for its ability to maintain mESC by comparison with commercial LIF as a control.
Results : The yield for the recombinant His6-hLIF was assessed to be approximately 1.7 mg from one liter of culture.
There were no statistically significant differences in expression of two pluripotency gene markers, oct-4 and Nanog, between mESCs treated with His6-hLIF and those with commercial hLIF (P = 0.09 and P = 0.13, respectively).
Besides, morphological characteristics (round cellular morphology) were similar between them.
Conclusions : Collectively, the findings showed that the ability of the recombinant His6-hLIF protein in maintaining pluripotent state of ESCs was comparable to commercial hLIF, providing evidence that the presence of the N-terminal hexahistidin tag does not influence biological activities of hLIF.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tahiri, Behrouz& Mudarrissi, Muhammad Husayn& Hashemitabar, Mahmud& Miryounesi, Muhammad& Mutivasili, Ilahi. 2018. Prokaryotic expression and purification of recombinant human leukemia inhibitory factor : analysis of the ability to maintain pluripotency in embryonic stem cells. Iranian Red Crescent Medical Journal،Vol. 20, no. 8, pp.1-6.
https://search.emarefa.net/detail/BIM-903572
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tahiri, Behrouz…[et al.]. Prokaryotic expression and purification of recombinant human leukemia inhibitory factor : analysis of the ability to maintain pluripotency in embryonic stem cells. Iranian Red Crescent Medical Journal Vol. 20, no. 8 (Aug. 2018), pp.1-6.
https://search.emarefa.net/detail/BIM-903572
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tahiri, Behrouz& Mudarrissi, Muhammad Husayn& Hashemitabar, Mahmud& Miryounesi, Muhammad& Mutivasili, Ilahi. Prokaryotic expression and purification of recombinant human leukemia inhibitory factor : analysis of the ability to maintain pluripotency in embryonic stem cells. Iranian Red Crescent Medical Journal. 2018. Vol. 20, no. 8, pp.1-6.
https://search.emarefa.net/detail/BIM-903572
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 5-6
رقم السجل
BIM-903572
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر