Solation and sequencing of mycoplasma arginini genome associated with respiratory disorder in small ruminants

الملخص EN

The purpose of this study was to evaluate the role and variety of Mycoplasma arginini (M.

arginini) in respiratory manifestation in sheep and goat flocks.

M.

arginine, were isolated from 40 nasal swabs out of 160 nasal swabs examined which obtained from (79) sheep and (81) goat with respiratory manifestations.

In sheep, the percent of isolation was (25.32%), 20 isolate out of 79 nasal swabs, while the percent of isolation in goat was (24.69%) 20 isolate out of 81 nasal swabs examined.

Higher percent of isolation was found in age group (1- 2) year old sheep (50%) as 7 isolates out of 14 nasal swabs examined, while in goat the higher percent of isolation was in age group less than one year by 44% (11 isolates out of 25 nasal swabs examined.

The lower isolation rates were found in adult sheep and goat (3 - 4 years old) by 15.38% in sheep and 6.45% in goat.

Mycoplasma isolates was molecularly characterized by PCR and DNA sequencing as M.

arginini and submitted to Gen Bank.

An accession number has been assigned to each nucleotide sequence and was KP972458-KP972459.