Molecular studies on some virulence factors of pseudomonas aeruginosa isolated from chickens as a biofilm forming bacteria
العناوين الأخرى
دراسات جزيئية على بعض عوامل الضراوة للسيدوموناس ايريجنوزا المعزولة من الدجاج كبكتيريا مكونة للبيوفيلم
المؤلفون المشاركون
Nabil, Nahhal Mahmud
Rida, Rim Muhammad
Tawakkul, Maram Muhammad Salah
المصدر
Assiut Veterinary Medical Journal
العدد
المجلد 64، العدد 159 (31 أكتوبر/تشرين الأول 2018)، ص ص. 43-51، 9ص.
الناشر
تاريخ النشر
2018-10-31
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
This study was aimed to isolate and identify Pseudomonas aeruginosa (P.
aeruginosa) from 150 diseased broiler chickens in addition to 50 environmental swabs from water pipes and tanks to examine their susceptibility against some commonly used antimicrobial agents, in addition to detection of some virulence genes using Polymerase Chain Reaction (PCR) and evaluation of its ability to form biofilm in vitro.
Clinically the affected chickens were subjected to postmortem examination (P.M) then samples from internal organs such as (liver, heart, lung, spleen, and intestine); tracheal and environmental swabs were collected and subjected to bacteriological examination and identification.
Twenty P.
aeruginosa isolates were recovered from the diseased chickens and environmental swabs with an incidence of (10%).
Sixteen isolates were isolated from internal organs of 150 diseased chickens with an incidence of (10.66%); meanwhile 4 isolates were recoded from 50 water pipes and tanks swabs with a percentage of (8%).
Antimicrobial sensitivity testing showed highly sensitivity to amikacin and colistin sulphate with percentages of (90%) for each of them.
Doxycycline showed resistance with a percentage of (75%).
PCR technique was a good tool for testing three virulence genes; pslA, pelA and fliC genes; the three genes were detected in of the examined samples with a percentage of (100%).
A significant relationship between the existence of three virulence genes in the isolated P.
aeruginosa and ability of biofilm formation (Slime producing ability) was reported in this study
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tawakkul, Maram Muhammad Salah& Nabil, Nahhal Mahmud& Rida, Rim Muhammad. 2018. Molecular studies on some virulence factors of pseudomonas aeruginosa isolated from chickens as a biofilm forming bacteria. Assiut Veterinary Medical Journal،Vol. 64, no. 159, pp.43-51.
https://search.emarefa.net/detail/BIM-909327
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tawakkul, Maram Muhammad Salah…[et al.]. Molecular studies on some virulence factors of pseudomonas aeruginosa isolated from chickens as a biofilm forming bacteria. Assiut Veterinary Medical Journal Vol. 64, no. 159 (Oct. 2018), pp.43-51.
https://search.emarefa.net/detail/BIM-909327
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tawakkul, Maram Muhammad Salah& Nabil, Nahhal Mahmud& Rida, Rim Muhammad. Molecular studies on some virulence factors of pseudomonas aeruginosa isolated from chickens as a biofilm forming bacteria. Assiut Veterinary Medical Journal. 2018. Vol. 64, no. 159, pp.43-51.
https://search.emarefa.net/detail/BIM-909327
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
رقم السجل
BIM-909327
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر