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A comparative study of bacterial diversity based on culturable and culture-independent techniques in the rhizosphere of maize (Zea mays L.)
المؤلفون المشاركون
Karim, Sajjad
Mirza, Zinat
Qaisrani, Tahir Batul
Qaisrani, Muthir Mansur
Zuhayr, Ahmad
Mirza, Muhammad Sajjad
Naqqash, Tahir
Hanif, Muhammad Kashif
Rasul, Ghulam
Malik, Kawthar Abd Allah
Allah, Salim
Jamal, Muhammad Sarwar
Rasul, Mahmud
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 26، العدد 7 (30 نوفمبر/تشرين الثاني 2019)، ص ص. 1344-1351، 8ص.
الناشر
تاريخ النشر
2019-11-30
دولة النشر
السعودية
عدد الصفحات
8
التخصصات الرئيسية
الموضوعات
الملخص EN
Objective Maize is an important crop for fodder, food and feed industry.
The present study explores the plant-microbe interactions as alternative eco-friendly sustainable strategies to enhance the crop yield.
Methodology Bacterial diversity was studied in the rhizosphere of maize by culture-dependent and culture-independent techniques by soil sampling, extraction of DNA, amplification of gene of interest, cloning of desired fragment and library construction.
Results Culturable bacteria were identified as Achromobacter, Agrobacterium, Azospirillum, Bacillus, Brevibacillus, Bosea, Enterobacter, Microbacterium, Pseudomonas, Rhodococcus, Stenotrophomonas and Xanthomonas genera.
For culture-independent approach, clone library of 16S ribosomal RNA gene was assembled and 100 randomly selected clones were sequenced.
Majority of the sequences were related to Firmicutes (17%), Acidobacteria (16%), Actinobacteria (17%), Alpha-Proteobacteria (7%), Delta-proteobacteria (4.2%) and Gemmatimonadetes (4.2%) However, some of the sequences (30%) were novel that showed no homologies to phyla of cultured bacteria in the database.
Diversity of diazotrophic bacteria in the rhizosphere investigated by analysis of PCR-amplified nifH gene sequence that revealed abundance of sequences belonging to genera Azoarcus (25%), Aeromonas (10%), Pseudomonas (10%).
The diazotrophic genera Azotobacter, Agrobacterium and Zoogloea related nifH sequences were also detected but no sequence related to Azospirillum was found showing biasness of the growth medium rather than relative abundance of diazotrophs in the rhizosphere.
Conclusion The study provides a foundation for future research on focussed isolation of the Azoarcus and other diazotrophs found in higher abundance in the rhizosphere.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Qaisrani, Muthir Mansur& Zuhayr, Ahmad& Mirza, Muhammad Sajjad& Naqqash, Tahir& Qaisrani, Tahir Batul& Hanif, Muhammad Kashif…[et al.]. 2019. A comparative study of bacterial diversity based on culturable and culture-independent techniques in the rhizosphere of maize (Zea mays L.). Saudi Journal of Biological Sciences،Vol. 26, no. 7, pp.1344-1351.
https://search.emarefa.net/detail/BIM-909977
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Qaisrani, Muthir Mansur…[et al.]. A comparative study of bacterial diversity based on culturable and culture-independent techniques in the rhizosphere of maize (Zea mays L.). Saudi Journal of Biological Sciences Vol. 26, no. 7 (Nov. 2019), pp.1344-1351.
https://search.emarefa.net/detail/BIM-909977
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Qaisrani, Muthir Mansur& Zuhayr, Ahmad& Mirza, Muhammad Sajjad& Naqqash, Tahir& Qaisrani, Tahir Batul& Hanif, Muhammad Kashif…[et al.]. A comparative study of bacterial diversity based on culturable and culture-independent techniques in the rhizosphere of maize (Zea mays L.). Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 7, pp.1344-1351.
https://search.emarefa.net/detail/BIM-909977
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 1350-1351
رقم السجل
BIM-909977
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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