Investigation of microsatellite instability BAT 25 and BAT 26 in breast cancer patients by conventional PCR

الملخص EN

Background: Breast cancer is the most common cancer among women worldwide, comprising 23% of the 1.1 million female cancers that newly diagnosed each year.

Aims: Investigate the existence of microsatellite instability (MSI) in breast cancer of patients.

Settings and Design: Fifty female patients with invasive ductal breast carcinoma (IDC) collected.

Inclusion criteria of subjects include female patients with diagnostic feature of breast cancer and age range 26-42 years old untreated with chemotherapy or hormonal therapy.

Methods and Material: DNA had be extracted from frozen tissue samples of breast cancer.

This protocol done according to the kit manufacture’s manual of QIAamp DNA Mini kit from Qiagen – USA.

All samples tested for MSI bysingleplex PCR reactions using two microsatellite markers BAT 25 and BAT 26.PCR achieved in a final volume of 50 μl and after thermal cycles, gel visualization performed.

Statistical analysis used: The significance of differences in proportions was analysed by the Fisher’s exact testwith SPSS version 20 and P values equal or less than 0.001 considered statistically significant.

Results: PCR demonstrating microsatellite instability in 13 (26%) of the 50 breast cancer sample.

Eight (16%) of 50 breast cancer sample were BAT 25 positive with a PCR product size of 124 bp, while 5 (10%) of 50 breast cancer sample were BAT 26 positive with a PCR product size 121bp.

Conclusions: The result suggest strong evidence that microsatellite instability (MSI) at the BAT 25 and BAT 26 and have involved in the pathogenesis of the great majority of breast cancers.