Antimicrobial susceptibility to salmonella and E.Coli isolates originated from broiler chickens in Luxor governorate

الملخص EN

Salmonellosis and colibacillosis are continuing serious problems facing poultry industry in Egypt.

In this study, 300 different pooled broiler chickens organs samples as (trachea, lung, liver, heart, spleen, unabsorbed yolk sac and intestine) were collected from different apparently healthy and sick broiler flocks in different localities of Luxor governorate during (2015 and 2016).

The examined broiler flocks were suffering from various health problems developed during the final two weeks of the growing period, resulting in increased mortality and condemnation losses.

Bacterial isolation was done by using standard method of isolation and identification.

The results showed that 92 out of 300 broiler organs samples were positive for Salmonella spp.

(30.66%) while 161 out of 300 were E.coli positive (53.66%).

The present study showed that the main Salmonella spp.

isolates were (S.

Typhimurium (24%), the both of S.

Enteritidis, S.

Anatum were (21.7%) and S.

Kentucky (19.56%), S.

Bargny and S.

Molade (3.26%) then S.

Newport, S.

Ingada and S.

Agona their percentage were (2.17%) respectively, while 8 serotypes of E.coli were obtained with the following serological identification O78 (44%), O1:H7 (17.39%), O91:H21 (15.52%), O128:H2 (13%) and other E.coli serotypes were identified as (O2:H6, O26:H11, O55:H7, O146:H21) their percentage was (1:3%).

Bacterial strains were tested against 21 antibacterial agents using the standard disk diffusion method on Muller and Hinton’s Agar medium.

The results were recorded that most of Salmonella spp were highly resistant to (Oxytetracycline, Doxycycline, Tetracycline then Enrofloxacin, Sulphamethoxazole) and were sensitive to (Gentamycin, colistinsulphate and Ceftiofur).

While most of E.coli isolates were resistant to Neomycin and Streptomycin and were sensitive to Ceftiofur then Colistinsulphate.