Expression and functional analysis of P450 gene induced tolerance resistance to lambda-cyhalothrin in quercetin fed larvae of beet armyworm Spodoptera exigua (Hubner)
المؤلفون المشاركون
Bashir, Muhammad Amjad
Ali, Sajjad
Yusuf, Hafiz Kamran
Waqas, Muhammad
Ahmad, Muhammad Afaq
Jan, Sad
Numan, Ali
Wang, Mo
Gharmh, Hamid A.
Qasim, Muhammad
Hafiz, Muhammad
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 27، العدد 1 (31 يناير/كانون الثاني 2020)، ص ص. 77-87، 11ص.
الناشر
تاريخ النشر
2020-01-31
دولة النشر
السعودية
عدد الصفحات
11
التخصصات الرئيسية
الموضوعات
الملخص EN
Beet armyworm, Spodoptera exigua (Hübner) is an agronomical important and most devastating polyphagous pest that damages a variety of crops around the globe including China.
Quercetin is one of the abundant dietary flavonoids and the important defense allelochemicals in plants.
Therefore, the changes in insect detoxification enzymes activities in response to plants allelochemicals may result increased the sensitivity to insecticides.
In this study, we examined the induced effect of quercetin on larval tolerance to lambda-cyhalothrin in S.
exigua.
Application of cytochrome P450 inhibitor piperonyl butoxide (PBO) significantly synergized the lambda-cyhalothrin toxicity in quercetin-fed S.
exigua larvae.
Moreover, larval weight significantly reduced in quercetin, lambda-cyhalothrin, and quercetin + lambda-cyhalothrin treatment.
Furthermore, our results showed that the P450 detoxification enzyme effectively increased in all treatments as compared to the control.
Quantitative Real-time PCR analysis revealed that expression level of CYP6AE10 significantly upregulated in larvae treated with quercetin, lambda-cyhalothrin and quercetin + lambda-cyhalothrin in the midgut and fat body respectively.
In addition, RNAi mediated knockdown of CYP6AE10 in S.
exigua larvae significantly decreased the transcription level of target cytochrome P450 gene followed by the exposure with quercetin, lambda-cyhalothrin, and quercetin + lambda-cyhalothrin.
Similarly, the knockdown of CYP6AE10 by the injection of dsRNA led to increased mortality after the treatment with respective chemicals.
Overall, these data showed that P450s might possibly play an important role in the metabolic adaptation of S.
exigua larvae to its host plant defense allelochemicals as well as insecticides.
In conclusion, S.
exigua can take benefit from its host plant’s secondary metabolites to elaborate its defense against synthetic insecticides.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hafiz, Muhammad& Qasim, Muhammad& Ali, Sajjad& Yusuf, Hafiz Kamran& Waqas, Muhammad& Ihsan, Ali…[et al.]. 2020. Expression and functional analysis of P450 gene induced tolerance resistance to lambda-cyhalothrin in quercetin fed larvae of beet armyworm Spodoptera exigua (Hubner). Saudi Journal of Biological Sciences،Vol. 27, no. 1, pp.77-87.
https://search.emarefa.net/detail/BIM-920463
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ihsan, Ali…[et al.]. Expression and functional analysis of P450 gene induced tolerance resistance to lambda-cyhalothrin in quercetin fed larvae of beet armyworm Spodoptera exigua (Hubner). Saudi Journal of Biological Sciences Vol. 27, no. 1 (Jan. 2020), pp.77-87.
https://search.emarefa.net/detail/BIM-920463
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hafiz, Muhammad& Qasim, Muhammad& Ali, Sajjad& Yusuf, Hafiz Kamran& Waqas, Muhammad& Ihsan, Ali…[et al.]. Expression and functional analysis of P450 gene induced tolerance resistance to lambda-cyhalothrin in quercetin fed larvae of beet armyworm Spodoptera exigua (Hubner). Saudi Journal of Biological Sciences. 2020. Vol. 27, no. 1, pp.77-87.
https://search.emarefa.net/detail/BIM-920463
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 85-87
رقم السجل
BIM-920463
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر