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Poloxamer-chitosan-based Naringenin nanoformulation used in brain targeting for the treatment of cerebral ischemia
المؤلفون المشاركون
Ahmad, Radwan
Ahmad, Niyaz
Ahmad, Farhan Jalees
Alam, Md Aftab
Amir, Mohd
Ali, Abuzer
Ahmad, Wasim
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 27، العدد 1 (31 يناير/كانون الثاني 2020)، ص ص. 500-517، 18ص.
الناشر
تاريخ النشر
2020-01-31
دولة النشر
السعودية
عدد الصفحات
18
التخصصات الرئيسية
الموضوعات
الملخص EN
Objective Here, the aim is to improve the bioavailability of Naringenin (NRG) in brain and to establish the highest remedial benefit from a novel anti-ischemic medicine i.e.
NRG.
Methods A novel Naringenin-loaded-nanoemulsion (NE)-(in situ)-gel (i.e.
thermoresponsive), was formulated with the help of Poloxamer-407 (20.0% w/v).
Chitosan (CS, 0.50% w/v) was used to introduce the mucoadhesive property of NE-(in situ)-gel and finally called as NRG-NE-gel + 0.50%CS.
A novel UHPLC-ESI-Q-TOF-MS/MS-method was optimized and used for NRG-NE-gel + 0.50%CS to quantify the Pharmacokinetic-(PK)-parameters in plasma as well as brain and to evaluate the cerebral ischemic parameters after MCAO i.e.
locomotor activity, grip strength, antioxidant activity, and quantity the infarction volume in neurons with the safety/toxicity of NRG-NE-gel + 0.50%CS after i.n.
administration in the rats.
Results The mucoadhesive potency and gelling temperature of NRG-NE-gel + 0.50%CS were observed 6245.38 dynes/cm2 and 28.3 ± 1.0 °C, respectively.
Poloxamer-407 based free micelles size was observed 98.31 ± 1.17 nm with PDI (0.386 ± 0.021).
The pH and viscosity of NRG-NE-gel + 0.50%CS were found to be 6.0 ± 0.20 and 2447 ± 24cp (at 35.0 ± 1.0 °C temperature), respectively.
An elution time and m/z NRG were observed 1.78 min and 270.97/150.96 with 1.22 min and m/z of 301.01/150.98 for Quercetin (IS) respectively.
Inter and intra %precision and %accuracy was validated 1.01–3.37% and 95.10–99.30% with a linear dynamic range (1.00 to 2000.00 ng/ml).
AUC0-24 of plasma & brain were observed 995.60 ± 24.59 and 5600.99 ± 144.92 (ng min/ml g) in the rats after the intranasal (i.n.) administration of NRG-NE-gel + 0.50%CS.
No toxicological response were not found in terms of mortalities, any-change morphologically i.e.
in the microstructure of brain as well as nasal mucosa tissues, and also not found any visual signs in terms of inflammatory or necrosis.
Conclusion Intranasally administered NRG-NE-gel + 0.50%CS enhanced the bioavailability of Naringenin in the brain.
In the cerebral ischemic rats, significantly improved the neurobehavioral activity (locomotor & grip strength) followed by antioxidant activity as well as infarction volume.
Finally, the toxicity studies carried out and established the safe nature of optimized-NRG-NE-gel + 0.50%CS.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ahmad, Niyaz& Ahmad, Radwan& Ahmad, Farhan Jalees& Ahmad, Wasim& Alam, Md Aftab& Amir, Mohd…[et al.]. 2020. Poloxamer-chitosan-based Naringenin nanoformulation used in brain targeting for the treatment of cerebral ischemia. Saudi Journal of Biological Sciences،Vol. 27, no. 1, pp.500-517.
https://search.emarefa.net/detail/BIM-920761
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ahmad, Niyaz…[et al.]. Poloxamer-chitosan-based Naringenin nanoformulation used in brain targeting for the treatment of cerebral ischemia. Saudi Journal of Biological Sciences Vol. 27, no. 1 (Jan. 2020), pp.500-517.
https://search.emarefa.net/detail/BIM-920761
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ahmad, Niyaz& Ahmad, Radwan& Ahmad, Farhan Jalees& Ahmad, Wasim& Alam, Md Aftab& Amir, Mohd…[et al.]. Poloxamer-chitosan-based Naringenin nanoformulation used in brain targeting for the treatment of cerebral ischemia. Saudi Journal of Biological Sciences. 2020. Vol. 27, no. 1, pp.500-517.
https://search.emarefa.net/detail/BIM-920761
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 516-517
رقم السجل
BIM-920761
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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