Identification and inoculation of fungal strains from Cedrus deodara rhizosphere involve in growth and alleviation of high nitrogen stress

المؤلفون المشاركون

Ahmad, Rafiq
al-Shaykh, Muhammad Sulayman
Ahmad, Uways
al-Dusari, Munirah A.
Abbasi, Arshad Mahmud
Jamil, Hafiz Muhammad Ansab
Irshad, Uthman
al-Araidh, Ibrahim A.
al-Ghamidi, Abd Allah Ahmad

المصدر

Saudi Journal of Biological Sciences

العدد

المجلد 27، العدد 1 (31 يناير/كانون الثاني 2020)، ص ص. 524-534، 11ص.

الناشر

الجمعية السعودية لعلوم الحياة

تاريخ النشر

2020-01-31

دولة النشر

السعودية

عدد الصفحات

11

التخصصات الرئيسية

الأحياء

الموضوعات

الملخص EN

Cedrus deodara is economically and ethnobotanically an important forest tree and is shown to be at decline in Northern areas of Pakistan in recent years mainly due to high concentration of Nitrogen in forests.

Ectomycorrhizal (ECM) association forming fungi enables the forest trees to develop optimally by absorbing water from the rhizosphere through their absorptive hyphae and by making available the nutrients by mobilization of N and P from the organic substrates.

This study was conducted to identify the ECM strains from C.

deodara rhizosphere and to analyse the impact of high N load on the C.

deodara seedlings to establish N critical load value for coniferous forests of Pakistan.

Six new fungal strains were identified from the rhizosphere of C.

deodara and were registered at GenBank (NCBI) as Emmia latemarginata strain ACE1, Aspergillus terreus strain ACE2, Purpureocillium lilacinum strain ACE3, Talaromyces pinophilus strain ACE4, A.

fumigatus strain ACE5 and T.

pinophilus strain ACE6 with accession numbers MH145426, MH145427, MH145428, MH145429, MH145430 and MH547115.

Four out of six isolated strains were inoculated with seedlings of C.

deodara singly and in consortium (CN) in combination with nitrogen load of 0 (C), 25 (T1), 50 (T2), 100 kg N ha−1 yr−1 (T3).

Agronomic, physiological and gene expression studies for ExpansinA4 (EXPA4) and Cystatins (Cys) were made to analyse the impact of fungal strains in relation to high N stress.

This study suggests a positive impact of T1 (25 kg N ha−1 yr−1) Nitrogen load and a negative impact of T3 (100 kg N ha−1 yr−1) on growth parameters and expression patterns of EXPA4 and Cys genes.

Peroxidase (POX) activity decreased in the order ACE5 > ACE2 > C > ACE3 > ACE1 > CN.

However, the results of Superoxide dismutase (SOD) showed decreasing trend in the order ACE5 > C > CN > ACE1 > ACE2 > ACE3.

Strain ACE3 was shown to have a positive impact on the seedlings in terms of growth, physiology and expression of genes.

Present study suggests that newly identified fungal strains showing positive impact on the growth and physiology of C.

deodara could be used for the propagation of this economically important plant in Pakistan after pathogenicity test.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Jamil, Hafiz Muhammad Ansab& Ahmad, Uways& Irshad, Uthman& al-Ghamidi, Abd Allah Ahmad& al-Shaykh, Muhammad Sulayman& al-Araidh, Ibrahim A.…[et al.]. 2020. Identification and inoculation of fungal strains from Cedrus deodara rhizosphere involve in growth and alleviation of high nitrogen stress. Saudi Journal of Biological Sciences،Vol. 27, no. 1, pp.524-534.
https://search.emarefa.net/detail/BIM-920794

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Jamil, Hafiz Muhammad Ansab…[et al.]. Identification and inoculation of fungal strains from Cedrus deodara rhizosphere involve in growth and alleviation of high nitrogen stress. Saudi Journal of Biological Sciences Vol. 27, no. 1 (Jan. 2020), pp.524-534.
https://search.emarefa.net/detail/BIM-920794

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Jamil, Hafiz Muhammad Ansab& Ahmad, Uways& Irshad, Uthman& al-Ghamidi, Abd Allah Ahmad& al-Shaykh, Muhammad Sulayman& al-Araidh, Ibrahim A.…[et al.]. Identification and inoculation of fungal strains from Cedrus deodara rhizosphere involve in growth and alleviation of high nitrogen stress. Saudi Journal of Biological Sciences. 2020. Vol. 27, no. 1, pp.524-534.
https://search.emarefa.net/detail/BIM-920794

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references : p. 533-534

رقم السجل

BIM-920794