Effect of BA, NAA, methyl jasmonate and mannitol on callus induction of periwinkle Plant (Catharanthus roseus L. cv. Heatwave Mix)‎ by In vitro culture

الملخص EN

The study was conducted to examine the role of BA, NAA, Methyl jasmonate and Mannitol in callus Induction.

The results showed the presence of significant differences between the treatments in the fresh and dry weight of callus after five weeks from culture.

The 0.25 mg.L-1 BA treatment was significantly superior on control treatment in fresh and dry weight of callus, which reached 0.293 and 0.152 mg, respectively.

Also, the two concentrations of NAA (0.5 and 1.0 mg.L-1) were significantly superior on control treatment in the same of two characteristics (0.218 and 0.272 mg fresh weight, and 0.099 and 0.152 mg dry weight, respectively).

The treatment of interaction between BA and NAA (0.25+1.0 mg.L-1) has given the highest significant difference in fresh and dry weight reached 0.444 and 0.269 mg, respectively.

While less fresh and dry weight when treatment was control treatment, which reached 0.0 mg.

The 8000 mg.L-1 Mannitol treatment was significantly superior on control treatment in fresh and dry weight of callus, which reached 332.42 and 53.81 mg, respectively.

Also, the 75 mg.L-1 concentration of Methyl jasmonate was significantly superior on control treatment in the same of two characteristics (347.19 and 55.67 mg fresh and dry weight).

The treatment of interaction between Mannitol and Methyl jasmonate(8000 mg.L-1+ 75 mg.L-1) has given the highest significant difference in fresh and dry weight reached 451.25 and 69.17 mg, respectively.

While less fresh and dry weight when treatment 0 mannitol + 25 mg.L-1 concentrations, which reached 211.99 and 25.38 mg, respectively.