Micropropagation and start codon targeted characterization of four stevia cultivars in Egypt
العناوين الأخرى
الأكثار الدقيق و التوصيف بإستخدام الواسمات المحددة لكودونات البداية فى أربعة أصناف من الاستيقيا في مصر
المؤلفون المشاركون
Abd al-Tawwab, Fathi Muhammad
Abd al-Hamid, Riham Ibrahim
Allam, Abd al-Wahhab Ismail
al-Dalifi, Ahmad al-Fatih
Abd al-Raziq, Ashraf Bakri
المصدر
Arab Universities Journal of Agricultural Sciences
العدد
المجلد 26، العدد 2 (31 ديسمبر/كانون الأول 2018)، ص ص. 635-645، 11ص.
الناشر
تاريخ النشر
2018-12-31
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الموضوعات
الملخص EN
Stevia rebaudiana Bertoni is a natural lowcalorie crop and commercially used as a noncaloric sweetener for diabetic patients.
It is also used as cosmetic ingredient, pickling agent, and dentifrice.
Four cultivars (Spantia, Shou2A3, China, and High Sugar) of stevia were included to optimize in vitro micropropagation.
Four different combinations of hormonal treatments were investigated [6-benzylamino purine (BAP) + Kinetin (Kin) (0.25 + 0.25 mg/l); Forchloefenuron (Cppu) + Kin (0.25 + 0.25 mg/l); Cppu+ Kin (0.5+0.25 mg/l); and the control medium (hormone-free)].
Out of the different media components, the hormone-free medium produced the best performance of explants.
The analysis of variance showed that the control treatment was the most significant for all traits except the number of branches per cutting.
Hardening of rooted plants was performed in plastic pots with 70% survival percentage during acclimatization.
Molecular characterization, of the four stevia cultivars, was conducted using 11 SCoT primers.
The SCoT analysis resulted in 122 amplicons, of which, 62 amplicons (51%) were polymorphic.
The range of polymorphism was between 6 % and 91 %.
The range of polymorphic amplicons per primer was between one and 12 amplicons.The SCoT-16 produced the highest number of polymorphic bands (12).
Meanwhile, the SCoT-24 produced the least polymorphism (6 %).
The current study provides a new micropropagation system with low cost, high efficiency, and hormonefree application.
Additionally, the study provides the first molecular characterization of stevia using SCoT marker system.
Finally, SCoT markers associated with cultivars having high and low contents of stevioside can further be validated by marker-assisted breeding studies
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abd al-Hamid, Riham Ibrahim& Abd al-Tawwab, Fathi Muhammad& Abd al-Raziq, Ashraf Bakri& Allam, Abd al-Wahhab Ismail& al-Dalifi, Ahmad al-Fatih. 2018. Micropropagation and start codon targeted characterization of four stevia cultivars in Egypt. Arab Universities Journal of Agricultural Sciences،Vol. 26, no. 2, pp.635-645.
https://search.emarefa.net/detail/BIM-938997
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Abd al-Hamid, Riham Ibrahim…[et al.]. Micropropagation and start codon targeted characterization of four stevia cultivars in Egypt. Arab Universities Journal of Agricultural Sciences Vol. 26, no. 2 (2018), pp.635-645.
https://search.emarefa.net/detail/BIM-938997
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abd al-Hamid, Riham Ibrahim& Abd al-Tawwab, Fathi Muhammad& Abd al-Raziq, Ashraf Bakri& Allam, Abd al-Wahhab Ismail& al-Dalifi, Ahmad al-Fatih. Micropropagation and start codon targeted characterization of four stevia cultivars in Egypt. Arab Universities Journal of Agricultural Sciences. 2018. Vol. 26, no. 2, pp.635-645.
https://search.emarefa.net/detail/BIM-938997
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
رقم السجل
BIM-938997
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر