In vitro evaluation the influence of glass-ceramic degradation products on osteoblast cells

الملخص EN

Regenerative medicine focuses on using biomaterials as three-dimensional (3D) porous scaffolds, specifically designed to mimic the nature of host tissue and hence to promote cell growth and tissue regeneration.

3D bioactive glass-ceramic scaffolds are one of the most frequently studied types of scaffolds for bone tissue engineering because of their excellent bioactivity and potential for stimulating osteogenesis and angiogenesis.

For such purposes, porous 3D 70%SiO2-30%CaO bioactive glass-ceramic scaffolds with three different pore sizes and identical porosity are used in present study to investigate In vitro, the effect of pore size on the degradation rate of scaffold which is achieved through examining changes in the composition of the immersion solution(SBF, simulated body fluid), and to investigate the action of released ions from the bioactive glass-ceramic scaffold during soaking process on osteoblast cells The results confirmed that all three scaffolds behaved in a similar manner and the ions release from the three scaffolds were of comparable concentration, which may be attributable to the identical porosity for all the scaffolds in addition to the using static immersion which delays ions diffusion.

The pH of culture media increased from 7.6 to 8.2 after one day soaking.

The optical microscopy images demonstrated that high ion concentration (Si, Ca, P) in the culture medium could have a negative effect on the cells and induce cell death, while low concentration of ionic dissolution products induces osteoblast proliferation in dilute culture medium