Occurrence and detection of Carbapenemase-Producing Klebsiella pneumoniae clinical isolates in Najaf Hospitals
المؤلفون المشاركون
al-Muhnah, Ali Muhsin Nimah
al-Sahlawi, Zuhayr Sadiq Razzaq
al-Dhahab, Azhar Umran Latif
المصدر
al-Kufa University Journal for Biology
العدد
المجلد 5، العدد 2 (31 ديسمبر/كانون الأول 2013)10ص.
الناشر
جامعة الكوفة كلية العلوم قسم علوم الحياة
تاريخ النشر
2013-12-31
دولة النشر
العراق
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Resistance to carbapenems is emerging, and it is a great problem to therapeutics.
Carbapenemase producing Klebsiella pneumoniae have been reported to be important cause of nosocomial infections.
There is no information available on carbapenemase producing K.
pneumoniae from Najaf.
The purpose of this study was undertaken to determine the occurrence of carbapenmase including metallo β-lactamases (MBLS), Klebsiella pnuemoniae carbapenemase (KPC) and GES-β-lactamase producing K.
pneumoniae.
A total of 770 clinical samples were collected from April to July, 2010.
The K.
pneumoniae isolates were identified according to API 20E system.
Phenotypic detection of carbapenemase was performed by using the imipenem-EDTA disk method and confirmed by the modified Hodg test.
KPC was detected on KPC CHROMagar medium.
The isolates were subjected to polymerase chain reaction (PCR) assays with specific primers for blaIMP, blaVIM blaKPC.
blaCTX-M and blaAmpC.
Only 108 (14.%) K.
pneumoniae isolates were recovered from clinical infections.
only 95 (88%) isolates were β-lactam resistant (resistant to both ampicillin and amoxicillin).
Of the β-lactam resistant isolates, 4/95 (4.2%) were found to be carbapenem disk resistant, of which,2 (50%) gave positive result with the imipenem-EDTA test, 4 (100%) gave positive result with modefied Hodg test and all isolates (100%) gave heavy growth on KPC CHROMagar.
In PCR experiments using specific primers for blaIMP, blaVIM and blaKPC genes, the results were negative among all isolates.
All isolate were harbored blaCTX-M and blaAmpC genes.
The present findings suggest that the spread of carbapenemases in isolates is high in Najaf.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Muhnah, Ali Muhsin Nimah& al-Dhahab, Azhar Umran Latif& al-Sahlawi, Zuhayr Sadiq Razzaq. 2013. Occurrence and detection of Carbapenemase-Producing Klebsiella pneumoniae clinical isolates in Najaf Hospitals. al-Kufa University Journal for Biology،Vol. 5, no. 2.
https://search.emarefa.net/detail/BIM-939713
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Sahlawi, Zuhayr Sadiq Razzaq…[et al.]. Occurrence and detection of Carbapenemase-Producing Klebsiella pneumoniae clinical isolates in Najaf Hospitals. al-Kufa University Journal for Biology Vol. 5, no. 2 (2013).
https://search.emarefa.net/detail/BIM-939713
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Muhnah, Ali Muhsin Nimah& al-Dhahab, Azhar Umran Latif& al-Sahlawi, Zuhayr Sadiq Razzaq. Occurrence and detection of Carbapenemase-Producing Klebsiella pneumoniae clinical isolates in Najaf Hospitals. al-Kufa University Journal for Biology. 2013. Vol. 5, no. 2.
https://search.emarefa.net/detail/BIM-939713
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-939713
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر