Measuring the viability of bacillus cereus bacteria on the biodegradation of crude oil in soil and incubated at varying temperatures and concentrations

الملخص EN

Forty-four isolates were isolated from soils of crude oil collected from different contaminated sites of crude oil refineries and reservoirs in Al-sulaymaniyah province on (MYP) medium.

They were found to be Bacillus cereus bacteria.

After phenotypic and biochemical tests, primary and secondary screening was performed on solid (BHM) medium covered with etheric solution of crude oil, 15 isolates were selected from the primary screening on the basis of growth density.

The isolate marked Bacillus cereus (B28) out of 15 isolates as the most efficient depending on the diameter highest rate of the translucent ring which reach (3.1cm).

the detection of isolation was confirmed by vitek2 device with detection kits BCL ID card.

Isolate viability (B28), which decomposes the hydrocarbons of crude oil, was studied by growing it on the liquid medium (BHM) that contains different concentrations for the two types of crude oil (average-kirkuk, heavy- Al-Qayyarah) (1,2,3%) and a different PH value (6,7,8).

It was the highest rate for the consumption and preparation of living cells for Kirkuk oil (36.2%) and (29.3x106 ) respectively within 144 hours and at concentration of (1%) v/v, PH (7) and temperature 35Co .