Ginger essential oil in vitro inhibits cell growth and induces apoptosis in mcf-7 human breast adenocarcinomacells
العناوين الأخرى
زيت الزنجبيل الطيار يثبط النمو ويحدث موت الخلايا المبرمج في خلايا سرطان الثدي MCF-7
المؤلفون المشاركون
Abd al-Rahman, Atif Abd al-Muhsin
Alimova, F.K.
Ulwan, Hamadah Abd al-Hamid Muhanni
al-Shafii, Sali Muhammad Abd al-Aziz
المصدر
Zagazig Journal for Agricultural Researches
العدد
المجلد 44، العدد 6 (31 ديسمبر/كانون الأول 2017)، ص ص. 2673-2683، 11ص.
الناشر
تاريخ النشر
2017-12-31
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Ginger plant (Zingiber officinale) is known as one of the important medicinal plant and dietary substances that are rich in phytochemicals.
It has been used as a food flavoring agent as well as for traditional oriental medicinal purposes for centuries.
Therefore, this study aimed to evaluate the inhibition of growth and induction of apoptosis (in vitro) in breast cancer cell line (MCF- 7) using ginger essential oil (GEO).
For this purpose, cells were exposed to 0, 20, 60 and 100 µg/ml of GEO for 72 hr.
Cell viability and cell proliferation were performed by using trypan blue and 3-(4,5- dimethylthiazol-2yl)- 2,5-biphenyl tetrazolium bromide (MTT) assays.
Cellular morphological changes were examined by phase contrast inverted microscopy.
Furthermore, the mitochondrial transmembrane potential assay was evaluated by flow cytometry using tetramethylrhodamine, ethyl ester (TMRE) dye as an indicator of apoptosis.
The results revealed that, human breast cancer cell line (MCF-7) was sensitive to the treatment with various GEO concentrations.
Treatment of cells with GEO resulted in a reduction in cell viability, growth inhibition, distinct changes in cellular morphology and induction of apoptosis in a concentration dependent manner.
The lowest percentage of viable cells was recorded as 20.68% at a concentration of 100 µg/ml of GEO.
GEO was found to have cytotoxic activity against MCF-7 cells (in vitro) with an IC50 value of 10.12 µg/ml and induces apoptosis by loss of mitochondrial membrane potential (∆Ψm).
Our findings demonstrate that, GEO inhibited MCF-7 proliferation by inducing apoptosis and may have applications in the field of anticancer drug development
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abd al-Rahman, Atif Abd al-Muhsin& al-Shafii, Sali Muhammad Abd al-Aziz& Ulwan, Hamadah Abd al-Hamid Muhanni& Alimova, F.K.. 2017. Ginger essential oil in vitro inhibits cell growth and induces apoptosis in mcf-7 human breast adenocarcinomacells. Zagazig Journal for Agricultural Researches،Vol. 44, no. 6, pp.2673-2683.
https://search.emarefa.net/detail/BIM-940633
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Abd al-Rahman, Atif Abd al-Muhsin…[et al.]. Ginger essential oil in vitro inhibits cell growth and induces apoptosis in mcf-7 human breast adenocarcinomacells. Zagazig Journal for Agricultural Researches Vol. 44, no. 6 (2017), pp.2673-2683.
https://search.emarefa.net/detail/BIM-940633
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abd al-Rahman, Atif Abd al-Muhsin& al-Shafii, Sali Muhammad Abd al-Aziz& Ulwan, Hamadah Abd al-Hamid Muhanni& Alimova, F.K.. Ginger essential oil in vitro inhibits cell growth and induces apoptosis in mcf-7 human breast adenocarcinomacells. Zagazig Journal for Agricultural Researches. 2017. Vol. 44, no. 6, pp.2673-2683.
https://search.emarefa.net/detail/BIM-940633
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
رقم السجل
BIM-940633
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر