Detection and differentiation of Entamoeba histolytica and Entamoeba dispar by enzyme linked immuno sorbent assay
العناوين الأخرى
الكشف والتمييز بين الاميبا النسيجية و الاميبا المتغيرة بواسطة الاليزا
المؤلف
المصدر
Kirkuk University Journal-Scientific Studies
العدد
المجلد 11، العدد 3 (30 سبتمبر/أيلول 2016)، ص ص. 263-277، 15ص.
الناشر
تاريخ النشر
2016-09-30
دولة النشر
العراق
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Amoebiasis is an important parasitic disease in human.
The two species Entamoeba histolytica and Entamoeba dispar are morphologically identical but E.
dispar is none pathogenic and is not associated with symptomatic amoebiasis.
In this study, from July to December 2013, 397 (212 male, 185 female) stool samples from in and out patients in Kirkuk Azady Teaching Hospital were examined microscopically.
97 samples of them were positive for Entamoeba histolytica / dispar.
Blood samples were collected from E.
histolytica / dispar positive patients, and the sera were examined by ELISA for differentiating the two species and evaluating the IgG levels in their serum.
The overall rate of E.
histolytica / dispar detected microscopically was 24.4%, while when the positive samples examined by ELISA technique 89.7% of them were E.
histolytica and 10.3% were considered to be E.
disbar.
The serum samples of 27.58% of the patients whom had E.
histolytica were positive for IgG antibody.
The most age group which was infected with E.
histolytica / dispar in both sexes were 41-50 years with rates of 39.13, 34.6 % for each of males and females respectively.
A significantly high frequency (62.9, 94.8 %) of E.
histolytica / dispar positive samples were contained RBC and pus cells respectively for each cell type, and the highest rate (28.8, 39.1%) were for those samples contained three pluses respectively for each of RBC and pus cells.
The conclusion is that there is a big necessity of a serology confirmatory test after microscopic detection of E.
histolytica to avoid un necessary treatment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ubayd, Hiru Muhammad. 2016. Detection and differentiation of Entamoeba histolytica and Entamoeba dispar by enzyme linked immuno sorbent assay. Kirkuk University Journal-Scientific Studies،Vol. 11, no. 3, pp.263-277.
https://search.emarefa.net/detail/BIM-944866
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ubayd, Hiru Muhammad. Detection and differentiation of Entamoeba histolytica and Entamoeba dispar by enzyme linked immuno sorbent assay. Kirkuk University Journal-Scientific Studies Vol. 11, no. 3 (Sep. 2016), pp.263-277.
https://search.emarefa.net/detail/BIM-944866
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ubayd, Hiru Muhammad. Detection and differentiation of Entamoeba histolytica and Entamoeba dispar by enzyme linked immuno sorbent assay. Kirkuk University Journal-Scientific Studies. 2016. Vol. 11, no. 3, pp.263-277.
https://search.emarefa.net/detail/BIM-944866
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
رقم السجل
BIM-944866
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر