The role of genistein and fish oil in molecular and biochemical disorders of osteoclastogenesis as a risk factor for osteoporosis induced by anastrozole in laboratory rats (Rattus norvegicus)‎

الملخص EN

In order to determine the toxic effects of anastrazol and its relationship to the incidence of osteoporosis, this study was conducted (56 females laboratory rats ) aged (3-4 month) divided into (7 groups) each group composed 8 female rats as follows: The first group was treated with a anastrazol (0.02 mg/1 kg body weight) for a 60 days.

The second group was treated with anstrazol (0.02 mg/1 kg body weight) and fish oil (3750 mg/1 kg of body weight) for 60 days.

The third group was treated with anstrazol (0.02 mg/1 kg body weight), and phytoestrogen (genstien) (20 mg/1 kg of body weight) for 60 days.

The fourth group was treated with anstrazol (0.02 mg/1 kg body weight) , fish oil (3750 mg/1 kg of body weight) and phytoestrogen (genstien) (20 mg/1 kg of body weight) for a 60 Day.

The fifth group is treated with a drug phytoestrogen (genstien) (20 mg/1 kg of body weight) for a 60-day.

The sixth group were treated with fish oil (3750 mg/1 kg of body weight) for a 60-day.

The seventh group is a group control are not treated by any drugs for a 60 days.

The biochemical results showed a significant decline in value of estrogen in the GI (43.925±2.895) compared with GII, GIII and GIV while significant improvement observed in the estrogen level of GII, GIII and GIV respectively (70.308±2.140), (67.42±3.812) and (74.15± 3.603) compared with the first group (43.925±2.895).

Also the GIV showed the optimal improvement in estrogen level (74.15±3.603), as well, the biochemical results showed significant increase in the level of the alkaline phosphatase in the GI which showed (190.02±3.98) compared with GII, GIII and GIV while showed significant improvement of alkaline phosphatase values in the GII, GIII and GIV respectively (180.57±1.

89), (181.37±5.

03), (176.

8±2, 41).In Real-Time PCR RANK in the first group showed up-regulation (12.157±1.59) compared with the second, third and fourth groups (5.50±90.601), (6.903±0.130) and (3.018±0.027) respectively.

The results also showed an up-regulation RANKL in Group I (8.458±0.766) compared with the second, third and fourth groups respectively (6.071±0.53), (4.970±0.228) and (3.398±0.114).

While the results showed an upregulation OPG in the second, third and fourth respectively (1.924±0.08), (1.014±0.064) and (5.567±1.419) compared in the first group (0.009±0.0076).We concluded that the derivatives like genestein and fish oil lead to ameliorate the toxic effect of anastrazole, also these derivatives may minimized the risk of osteoporosis caused by estrogen deficiency