Simultaneous determination of Gallic acid and ascorbic acid using first derivative Zero-crossing spectrophotometric technique

الملخص EN

A new spectrophotometric method was used for simultaneous determination of gallic acid and ascorbic acid depending on the first derivative zero-crossing technique.

The first derivative spectra of these compounds permitted simultaneous determination of gallic acid and ascorbic acid over the concentration ranges of (1.0 – 30.0) μg/mL and (3.0 – 35.0) μg/mL with detection limits of 0.559 and 0.991 for gallic acid and ascorbic acid, respectively, by techniques of measuring the amplitude of peak-to-base line and zero-crossing at certain wavelengths.

The proposed method showed reasonable precision and accuracy and have been applied to determine gallic acid and ascorbic acid in samples of Hibiscus sabdariffa L.

KEY WORDS: Hibiscus sabdariffa L, Gallic acid, Ascorbic acid, Derivative spectrophotom