The effect of ethanol on some ecological and functional aspects of Dictyostelium discoideum beta-glucosidase

الملخص EN

Dictyostelium discoideum has revealed as appropriate tool for studying the effect of many ecological factors.

Dictyostelium can live in many places such as soil and moist leaf litter.

Beta- glucosidase is one of the most important enzymes that used in degradation of food and takes part in other cell biology.

Ethanol is a solvent that used widely rang for water insoluble substances in industry and medicine.

In this study, the ecological effects of ethanol and its cellular role on D.

discoideum beta-glucosidase were investigated.

Cell line was generated for over-expressing beta- glucosidase protein and cell line with the first 83 amino acid from beta- glucosidase (a mutant version of this protein) was used.

Beta- glucosidase overexpression cell line showed high level of growth rate, cells size, phototaxis and aggregation comparing to other studies cell lines even under high concentration of ethanol.

These data support a role for Betaglucosidase in resistance against the ecological factors like ethanol.