Dictyostelium discoideum paracaspase functions in secretion of lysosomal enzymes

الملخص EN

Dictyostelium discoideum has proven as useful tool in studying lysosomal membrane trafficking.

In Dictyostelium, lysosomes connect two main membrane trafficking pathways including the endocytic and the phagocytic pathway.

Paracaspase, a caspases- like protein discovered in Dictyostelium, has been implemented in trafficking roles along these pathways.

In this study, the cellular role of D.

discoideum paracaspase was investigated.

Cell line was generated that over-expressing paracaspase protein.

In addition, cell line with knockout pcp was used.

Both cell lines show over-secreted lysosomal enzymes, especially β- glucosidase, galactosidase and acid phosphatase.

These data support a role for paracaspase in trafficking along the lysosomal enzyme secretion which in turn effect on the endocytic, and phagocytic pathways.