Diagnostic and epidemiological study of Toxoplasma gondii for students of Thi-Qar University by ELISA and Real-Time PCR techniques
العناوين الأخرى
دراسة تشخيصية و وبائية لطفيلي المقوسة الكوندية Toxoplasma gondii لطلبة جامعة ذي قار باستعمال تقنيتي الإليزا و تفاعل سلسلة البلمرة ذو الوقت الحقيقي
المؤلفون المشاركون
al-Musawi, Rabab Ali Shinnawh
al-Abbudi, Bassad Aqrab
al-Tamimi, Manal Badi Salih
المصدر
Iraqi Journal of Biotechnology
العدد
المجلد 14، العدد 2 (31 ديسمبر/كانون الأول 2015)، ص ص. 295-311، 17ص.
الناشر
جامعة بغداد معهد الهندسة الوراثية و التقنيات الإحيائية للدراسات العليا
تاريخ النشر
2015-12-31
دولة النشر
العراق
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
Toxoplasmosis is caused by an obligate intracellular tissue protozoan parasite Toxoplasma gondii, which is able to infect humans as well as other warm blooded domestic and wild animals.
The infection has a world-wide distribution with approximately one-third of the world population estimated to be exposed to this parasite The present study was performed for the first time in Thi-Qar province to estimate the prevalence of toxoplasmosis among the university students.
Blood samples were collected from 319 (111 males and 208 females) apparently healthy students, they have ages between (18-42) years attended from different colleges of Thi-Qar university (students of morning and evening study), during the period from October 2013 to April 2014.
Enzyme linked Immunosorbant Assay (ELISA), was used to evaluate the presence of anti-Toxoplasma IgM and IgG antibodies and detection of B1 gene of T.gondii DNA by Real-Time Polymerase Chain Reaction (RT-PCR).
The results indicated that 21.94% of students were anti-Toxoplasma antibodies using ELISA test.
The study demonstrated that no significant difference in infection percentages between colleges where students studied, although the highest seropositivity students was 47.83% in Education Sports College whereas it did not score any infection with toxoplasmosis in College of Education for Pure Science .
The current study also showed that the highest positive percentage with T.gondii of male students was 28.57% in College of Engineering while the highest percentage with T.gondii of female students was 30.43% in College of sports education.
The present study revealed that the acute infection of toxoplasmosis which presented IgM and both IgM and IgG was high at small age young students (18-22) years while the chronic infection which presented IgG was high at old students (38-42) years.
The present study demonstrated that seroprevalence of toxoplasmosis was significantly associated with close contact with cats.
The present study revealed an association between blood group system and Toxoplasma infection with highest prevalence among samples of blood group AP+P 30.65% and lowest prevalence in samples of blood group BP+P 17.44%.
Besides the serological diagnosis of T.gondii, Real-Time PCR (RT-PCR) technique was used to confirm the infection with T.gondii by detection B1 gene of T.gondii DNA in the blood of students.
Out of 319 students only 6.26% showed positive for toxoplasmosis among those 2.50% were males and 3.76% were females.
The positive result in RT-PCR analysis were distributed on the patterns of the antiToxoplasma antibodies by ELISA test , Any positive blood samples with IgM, IgG and both IgM and IgG respectively have not been found whereas 20 positive cases of no anti –Toxoplasma antibodies.
Real-Time PCR test in student's blood has advantages in detection of recent or active toxoplasmosis
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Musawi, Rabab Ali Shinnawh& al-Abbudi, Bassad Aqrab& al-Tamimi, Manal Badi Salih. 2015. Diagnostic and epidemiological study of Toxoplasma gondii for students of Thi-Qar University by ELISA and Real-Time PCR techniques. Iraqi Journal of Biotechnology،Vol. 14, no. 2, pp.295-311.
https://search.emarefa.net/detail/BIM-955965
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Musawi, Rabab Ali Shinnawh…[et al.]. Diagnostic and epidemiological study of Toxoplasma gondii for students of Thi-Qar University by ELISA and Real-Time PCR techniques. Iraqi Journal of Biotechnology Vol. 14, no. 2 (2015), pp.295-311.
https://search.emarefa.net/detail/BIM-955965
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Musawi, Rabab Ali Shinnawh& al-Abbudi, Bassad Aqrab& al-Tamimi, Manal Badi Salih. Diagnostic and epidemiological study of Toxoplasma gondii for students of Thi-Qar University by ELISA and Real-Time PCR techniques. Iraqi Journal of Biotechnology. 2015. Vol. 14, no. 2, pp.295-311.
https://search.emarefa.net/detail/BIM-955965
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p.307-311
رقم السجل
BIM-955965
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر